Naturally occurring clones of cells with high intrinsic proliferation potential within the follicular epithelium of mouse thyroids
The proliferation pattern of some scattered clones of naturally occurring follicular cells with an exceedingly high intrinsic growth potential was investigated in the mouse thyroid gland. In particular, evidence was sought to demonstrate that the high propensity to replicate is a stable trait transmitted from the progenitor cells to their offspring. We hypothesize that these cell clones are at the origin of the multiple adenomas that invariably arise in chronically stimulated thyroid. Growth stimulation was induced either by hemithyroidectomy or by methimazole feeding. In a first series of experiments, involving hemithyroidectomized animals, (/sup 3/H)thymidine was administered continuously for 3 weeks by means of osmotic minipumps, so that all cells entering the mitotic cycle during that time were labeled. Hemithyroidectomy led to a 3-fold increase of the fraction of labeled cells in the remaining lobe. The increase was prevented by thyroxine treatment in thyroid-stimulating hormone-suppressing doses. Autoradiographs of contiguous serial sections across whole follicles showed that roughly 75% of the labeled cells were clustered in groups of 3 or more, rather than being randomly distributed. In a second set of experiments, glands stimulated by methimazole-induced thyroid-stimulating hormone hypersecretion were pulse-labeled by a single i.p. injection of (/sup 3/H)thymidine. Animals were sacrificed either 2 h or 3 weeks after the administration of the label. The thyroids were excised and the fate of labeled thyroid cells was analyzed autoradiographically.
- Research Organization:
- University Hospital, Linkoeping, Sweden
- OSTI ID:
- 6590077
- Journal Information:
- Cancer Res.; (United States), Vol. 6
- Country of Publication:
- United States
- Language:
- English
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CLONE CELLS
CELL PROLIFERATION
THYROID
ADENOMAS
AUTORADIOGRAPHY
CELL DIVISION
EPITHELIUM
MICE
THYMIDINE
THYROIDECTOMY
THYROXINE
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
AZINES
BODY
CARBOXYLIC ACIDS
CELL CULTURES
DISEASES
ENDOCRINE GLANDS
GLANDS
HETEROCYCLIC COMPOUNDS
HORMONES
LABELLED COMPOUNDS
MAMMALS
MEDICINE
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PYRIMIDINES
RIBOSIDES
RODENTS
SURGERY
THYROID HORMONES
TISSUES
VERTEBRATES
550901* - Pathology- Tracer Techniques