A rapid procedure to detect in situ DNA/RNA hybrids

Journal Article · Tue Dec 15 00:00:00 EST 1981 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(81)90903-7· OSTI ID:6589085

Reddy, A R ^[1]; Sofer, W

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1. Univ. of Hyderabad, India

A new method was developed for detecting DNA/RNA hydrids formed in situ using anti-DNA/RNA antibodies and the Peroxidase-antiperoxidase immunohistochemical procedure. Using RNA synthesized in vitro from cloned Drosophila histone genes (pDm 500H), we localized by this procedure, the histone genes to the 39 D-E region of the left arm of the second chromosome. This method has several advantages compared to conventional procedures.

• DOE Contract Number: AC02-76EV02965
• OSTI ID: 6589085
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 103:3
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
DNA
BIOASSAY
CYTOCHEMISTRY
HYBRIDIZATION
DROSOPHILA
GENETIC MAPPING
RNA
ANTIBODIES
HISTONES
IN VITRO
ANIMALS
ARTHROPODS
BIOCHEMISTRY
CHEMISTRY
DIPTERA
FLIES
FRUIT FLIES
INSECTS
INVERTEBRATES
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
500200* - Environment
Atmospheric- Chemicals Monitoring & Transport- (-1989)
500400 - Environment
Atmospheric- Thermal Effluents Monitoring & Transport- (-1989)