Endosperm protein synthesis and L-(/sup 35/S)methionine incorporation in maize kernels cultured in vitro
This study was conducted to examine protein synthesis and L-(/sup 35/S)methionine incorporation into the endosperm of Zea mays L. kernels developing in vitro. Two-day-old kernels of the inbred line W64A were placed in culture on a defined medium containing 10 microCuries L-(/sup 35/S)methionine per milliliter (13 milliCuries per millimole) and harvested at 10, 15, 20, 25, 30, 35, and 40 days after pollination. Cultured kernels attained a final endosperm mass of 120 milligrams compared to 175 milligrams for field-grown controls. Field and cultured kernels had similar concentrations (microgram per milligram endosperm for total protein, albumin plus globulin, zein, and glutelin fractions at most kernel ages. Sodium, dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing patterns for endosperm proteins were similar for field and cultured kernels throughout development. By 15 days, over 70% of the L-(/sup 35/S)methionine taken up was present in endosperm proteins. Label incorporation visualized by fluorography generally followed the protein intensity of the stained gels. The high methionine content, low molecular weight zeins (i.e. 15 and 9 kilodaltons) were highly labeled. All of the radioactivity in hydrolyzed zein samples was recovered in the methionine peak indicating minimal conversion to L-(/sup 35/S)cysteine. The procedure described here is suitable for long term culture and labeling experiments in which continued kernel development is required.
- Research Organization:
- Univ. of Minnesota, St. Paul
- OSTI ID:
- 6584028
- Journal Information:
- Plant Physiol.; (United States), Vol. 74:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ENDOSPERM
PROTEINS
METHIONINE
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CYSTEINE
ELECTROPHORESIS
IN VITRO
LABELLING
MAIZE
POLYACRYLATES
SEEDS
SODIUM
SULFATES
SULFUR 35
TRACER TECHNIQUES
ALKALI METALS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CEREALS
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
ESTERS
EVEN-ODD NUCLEI
GRAMINEAE
GRASS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPOTROPIC FACTORS
METALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PLANT TISSUES
PLANTS
POLYMERS
POLYVINYLS
RADIOISOTOPES
REACTION KINETICS
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
THIOLS
TISSUES
550201* - Biochemistry- Tracer Techniques