Study of the metabolism of /sup 13/C labeled substrates by /sup 13/C NMR spectroscopy of intact cells, tissues, and organs

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Am. Chem. Soc., Div. Pet. Chem., Prepr.; (United States)

OSTI ID:6583530

Matwiyoff, N A; London, R E; Hutson, J Y

Carbon-13 nuclear magnetic resonance spectroscopy, in conjunction with carbon-13 labeling, has become an important analytical technique for the study of biological systems and biologically important molecules. The growing list of its well established applications to isolated molecules in solution includes the investigation of: metabolic pathways; the microenvironments of ligands bound to proteins; the architecture and dynamics of macromolecules; the structures of coenzymes and other natural products; and the mechanisms of reactions. Recently interest has been reawakened in the use of the technique for the study of metabolic pathways and structural components in intact organelles, cells, and tissues. The promise and problems in the use of /sup 13/C labeling in such investigations can be illustrated by the results on suspensions of the yeast, Candida utilis.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 6583530
• Journal Information: Am. Chem. Soc., Div. Pet. Chem., Prepr.; (United States), Vol. 191
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CANDIDA
NUCLEAR MAGNETIC RESONANCE
CELL MEMBRANES
GLUCOSE
METABOLISM
GLUTAMIC ACID
CARBON 13
CHOLINE
ALCOHOLS
ALDEHYDES
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
CARBOHYDRATES
CARBON ISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DRUGS
EVEN-ODD NUCLEI
FUNGI
HEXOSES
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAGNETIC RESONANCE
MEMBRANES
MICROORGANISMS
MONOSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
QUATERNARY COMPOUNDS
RESONANCE
SACCHARIDES
STABLE ISOTOPES
YEASTS
550501* - Metabolism- Tracer Techniques
550701 - Microbiology- Tracer Techniques