Radioiodination of interleukin 2 to high specific activities by the vapor-phase chloramine T method

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(88)90477-0· OSTI ID:6583406

Siekierka, J J; DeGudicibus, S

Recombinant human interleukin 2 (IL-2) was radioiodinated utilizing the vapor phase chloramine T method of iodination. The method is rapid, reproducible, and allows the efficient radioiodination of IL-2 to specific activities higher than those previously attained with full retention of biological activity. IL-2 radioiodinated by this method binds with high affinity to receptors present on phytohemagglutinin-stimulated peripheral blood lymphocytes and should be useful for the study of receptor structure and function.

• Research Organization: Merck, Sharp and Dohme Research Laboratories, Rahway, NJ (USA)
• OSTI ID: 6583406
• Journal Information: Anal. Biochem.; (United States), Vol. 172:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LYMPHOKINES
IODINATION
RECEPTORS
STRUCTURE-ACTIVITY RELATIONSHIPS
BIOCHEMICAL REACTION KINETICS
CHLORAMINES
IODINE ISOTOPES
LABELLING
LYMPHOCYTES
MAN
MICE
TRACER TECHNIQUES
AMINES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
HALOGENATION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MITOGENS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PRIMATES
PROTEINS
REACTION KINETICS
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques