Trace analysis of doxylamine succinate in animal feed, human urine, and wastewater by GC using a rubidium-sensitized nitrogen detector

Journal Article · Sun Aug 01 00:00:00 EDT 1982 · J. Chromatogr. Sci.; (United States)

DOI:https://doi.org/10.1093/chromsci/20.8.373· OSTI ID:6576622

Thompson, Jr, H C; Holder, C L; Bowman, M C

Doxylamine succinate, a drug used as a sleep-inducing agent, an antihistamine, and in a therapeutic formulation taken by pregnant women as an antinauseant, was scheduled for toxicological evaluation as part of a structure activity relationship study, with rats and mice, because a deficiency of such data exists with regard to many antihistamines. Analytical chemical procedures that ensure proper concentration, homogeneity, and stability of the drug in dosed feed, as well as the safety of personnel and the environment, were prerequisites for the toxicological tests. GC methods using a rubidium-sensitized nitrogen detector were developed for analysis of doxylamine succinate in animal feed, human urine, and wastewater at levels as low as 1 ppm, 100 ppb, and 100 ppb, respectively. Sample extracts were cleaned up by liquid-liquid partitioning, followed by additional cleanup on a column of silica gel. Data are presented concerning the stability of the drug in animal feed, extraction efficiencies, and the use of the silica gel cleanup column to separate the caffeine interference from doxylamine in extracts of human urine. Partition values and ancillary data concerning analysis of the drug in feed, by HPLC at levels as low as 10 ppm, are also reported.

• Research Organization: Department of Health and Human Services, Food and Drug Administration, for Toxicological Research, Jefferson, Arkansas
• OSTI ID: 6576622
• Journal Information: J. Chromatogr. Sci.; (United States), Vol. 20:8
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ANIMAL FEEDS
ANTIHISTAMINICS
CHEMICAL ANALYSIS
TOXICITY
URINE
WASTE WATER
AMINES
CAFFEINE
CHROMATOGRAPHY
GAS CHROMATOGRAPHY
MAN
RUBIDIUM
SUCCINIC ACID
ALKALI METALS
ANALEPTICS
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM AGENTS
DICARBOXYLIC ACIDS
DRUGS
ELEMENTS
FOOD
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
LIQUID WASTES
MAMMALS
MATERIALS
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXYGEN COMPOUNDS
PRIMATES
PURINES
SEPARATION PROCESSES
VERTEBRATES
WASTES
WATER
XANTHINES
560306* - Chemicals Metabolism & Toxicology- Man- (-1987)
550200 - Biochemistry