Macromolecular crystallographic results obtained at CHESS using a detector incorporating a charge-coupled device

Journal Article · Wed Feb 01 00:00:00 EST 1995 · Review of Scientific Instruments; (United States)

DOI:https://doi.org/10.1063/1.1145946· OSTI ID:6575932

Thiel, D J; Walter, R L; Ealick, S E ^[1]; Bilderback, D H ^[2]; Tate, M W; Gruner, S M ^[3]; Eikenberry, E F ^[4]

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1. Section of Biochemistry, Cell and Molecular Biology, Cornell University, Ithaca, New York 14853 (United States)
2. Cornell High Energy Synchrotron Source (CHESS), Cornell University, Ithaca, New York 14853 (United States)
3. Department of Physics, Princeton University, Princeton, New Jersey 08544 (United States)
4. Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 (United States)

Results from various macromolecular crystallography experiments are presented showing the effectiveness of a recently installed detector incorporating a charge-coupled device (CCD). This detector uses a 1024[times]1024 CCD directly coupled to an x-ray sensitive phosphor by a fiber optic taper. The pixel size at the phosphor (50 [mu]m) results in a point spread of 80 [mu]m full width at half-maximum. Even with the relatively small active area, 51[times]51 mm[sup 2], about 150 orders of diffraction can be resolved across the detector face. With this detector format, well-resolved diffraction data have been collected from unit cells with edges as large as 360 A. In an offset configuration, the detector has been used to collect extremely high-resolution data (1 A). A number of data sets have been collected having [ital R][sub sym] values in the 4%--6% range; in the case of room-temperature lysozyme, an [ital R][sub sym] value as small as 2.1 was obtained for a 2.5 A resolution data set. In addition to fixed wavelength studies, the detector has also been used to collect MAD data. In all cases, the use of this detector has proven to be more efficient than using standard image plates since less x-ray exposure time and no distinct scanning step are required. Furthermore, the data quality is as good and in some cases better than those from previous image plate measurements.

• OSTI ID: 6575932
• Journal Information: Review of Scientific Instruments; (United States), Vol. 66:2; ISSN 0034-6748
• Country of Publication: United States
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
36 MATERIALS SCIENCE
MOLECULAR CRYSTALS
CRYSTAL STRUCTURE
X-RAY DIFFRACTOMETERS
CHARGE-COUPLED DEVICES
ENZYMES
PROTEINS
SYNCHROTRON RADIATION SOURCES
VIRUSES
CRYSTALS
DIFFRACTOMETERS
MEASURING INSTRUMENTS
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
RADIATION SOURCES
SEMICONDUCTOR DEVICES
665100* - Nuclear Techniques in Condensed Matter Physics - (1992-)
360602 - Other Materials- Structure & Phase Studies