Deletion mapping of chr. 16q in breast cancer using dual color FISH with highly efficient cosmid contig probes
- Univ. of California, San Francisco (United States)
- Los Alamos National Lab., NM (United States)
Breast cancer is known to have frequent allelic loss on the long arm of chr.16. To facilitate identification of genes from 16q involved in breast cancer progression, the authors performed dual color FISH with chr. 16 centromeric reference probe (detected in red) and locus-specific cosmid contigs (in green) distributed along chr.16q in order to define the region of common deletion. Six breast cancer cell lines and 23 primary breast cancers were examined. Cells were scored as carrying a deletion when the fraction of cells showing fewer locus specific signals than centromeric signals plus the fraction of cells showing less than two locus specific signals was greater than 0.5. Five of 6 breast cancer cell lines showed deletion on chr.16q. The region around 16q24 was deleted in 4 of 5 cell lines. Breast cancer cases were preselected on the basis of p53 deletion by FISH because of the high correlation between p53 deletion and 17q deletion. Chr.16q deletions were found in 13 cases (56%); 10 cases (43%) with the break point between the centromere and 17q12, and 3 cases (13%) with the break point between 16q13 and 16q21. Intriguingly, the common region of deletion in clinical breast cancer, 16q22 to q24, appears to include the locus (16q22.1) for E-cadherin gene encoding cell adhesion protein which might be associated with progression of breast cancer.
- OSTI ID:
- 6574623
- Report Number(s):
- CONF-9303114-; CODEN: CYTODQ
- Journal Information:
- Cytometry (Baltimore); (United States), Vol. 6; Conference: 16. congress of the International Society for Analytical Cytology, Colorado Springs, CO (United States), 21-26 Mar 1993; ISSN 0196-4763
- Country of Publication:
- United States
- Language:
- English
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