Degradation of connective tissue matrices by macrophages. II. Influence of matrix composition on proteolysis of glycoproteins, elastin, and collagen by macrophages in culture
- Univ. of Southern California, Los Angeles
Thioglycollate-elicited mouse peritoneal macrophages were cultured in contact with the mixture of extracellular matrix proteins produced by rat smooth muscle cells in culture. Both live macrophages and their conditioned media hydrolyzed glycoproteins, elastin, and collagen. Live macrophages also degraded extracellular connective tissue proteins secreted by endothelial cells and fibroblasts. The glycoproteins in the matrix markedly inhibited the rate of digestion of the other macromolecules, particularly elastin. When plasminogen was added to the matrix, activation of plasminogen to plasmin resulted in the hydrolysis of the glycoprotein components, which then allowed the macrophage elastase easier access to its substrate, elastin. Thus, although plasmin has no direct elastinolytic activity, its presence accelerated the rate of hydrolysis of elastin and therefore the rate of matrix degradation. These findings may be important in an understanding of disease states, such as emphysema and atherosclerosis, that are characterized by the destruction of connective tissue.
- DOE Contract Number:
- AC03-76SF01012
- OSTI ID:
- 6571124
- Journal Information:
- J. Exp. Med.; (United States), Vol. 152
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACTINOMYCIN
BIOLOGICAL EFFECTS
COLLAGEN
BIODEGRADATION
CONNECTIVE TISSUE
CHEMICAL COMPOSITION
CYCLOHEXIMIDE
FIBRINOLYSIN
GLUCOPROTEINS
TRYPSIN
ANIMAL CELLS
CELL CULTURES
MACROPHAGES
MORPHOLOGY
MUSCLES
PROLINE
PROTEOLYSIS
RATS
STIMULATION
TRITIUM COMPOUNDS
AMINES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AZOLES
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DECOMPOSITION
DRUGS
ENZYMES
FIBRINOLYTIC AGENTS
FUNGICIDES
HEMATOLOGIC AGENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HYDROLASES
PESTICIDES
PHAGOCYTES
PROTEINS
PYRROLES
PYRROLIDINES
RODENTS
SACCHARIDES
SCLEROPROTEINS
SERINE PROTEINASES
SOMATIC CELLS
TISSUES
VERTEBRATES
550200* - Biochemistry
550300 - Cytology