Adenosine 3',5'-monophosphate waves in dictyostelium discoideum: a demonstration by isotope dilution-fluorography
The distribution of adenosine 3',5'-monophosphate (cyclic AMP) in fields of aggregating amoebae of Dictyostelium discoidenum was examined by a novel isotope dilution-fluorographic technique. Cellular cyclic AMP was visualized by its competition with exogenous /sup 3/H-labeled cyclic AMP for high-affinity binding sites on protein kinase immobilized on a Millipore filter used to blot the monolayer. The cyclic AMP was distributed in spiral or concentric circular wave patterns which centered on the foci of the aggregations. These patterns were correlated with those of cell shape change that propagate through the monolayers. These observations support the hypothesis that the aggregation process in Dictyostelium is mediated by the periodic relay of cyclic AMP signals and suggest a simple scheme for the dynamics of the aggregation process.
- Research Organization:
- Univ. of Chicago, IL
- OSTI ID:
- 6566929
- Journal Information:
- Science; (United States), Vol. 212
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AGGLOMERATION
CORRELATIONS
AMOEBA
MORPHOLOGY
AMP
FLUORESCENCE
ISOTOPE DILUTION
TRITIUM COMPOUNDS
ANIMALS
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUMINESCENCE
MICROORGANISMS
NUCLEOTIDES
ORGANIC COMPOUNDS
PROTOZOA
SARCODINA
TRACER TECHNIQUES
550701* - Microbiology- Tracer Techniques