In vitro studies of interaction of rickettsia and macrophages: effect of ultraviolet light on Coxiella burnetti inactivation and macrophage enzymes

Journal Article · Sat Mar 01 00:00:00 EST 1980 · Infect. Immun.; (United States)

OSTI ID:6566552

Little, J S; Kishimoto, R A; Canonico, P G

The inactivation of Coxiella burnetii in suspension or in cultures of guinea pig peritoneal macrophages by ultraviolet (uv) light was studied. The effect of uv treatment on the activity of macrophage organelle marker enzymes and their subsequent equilibration in linear sucrose gradients was also determined. It was shown that uv treatment for 15 s at a distance of 10 cm inactivated C. burnetti, either in suspension or within guinea pig peritoneal macrophages. Similar uv treatment had little effect on the activity or equilibration of macrophage organelle marker enzymes in linear sucrose gradients.

• Research Organization: Army Medical Research Inst. of Infectious Diseases, Fort Detrick, Frederick, MD
• OSTI ID: 6566552
• Journal Information: Infect. Immun.; (United States), Vol. 27:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ENZYME ACTIVITY
BIOLOGICAL RADIATION EFFECTS
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INACTIVATION
IN VITRO
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560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)