Relationship between X-ray exposure and malignant transformation in C3H 10T1/2 cells
- Harvard Univ., Boston, MA
The appearance of transformed foci after x-irradiation of the C3H 10T1/2 line of murine cells requires extensive proliferation followed by prolonged incubation under conditions of confluence. When the progeny of irradiated cells are resuspended and plated to determine the number of potential transformed foci, the absolute yield is constant over a wide range of dilutions and is similar to that observed in cultures that have not been resuspended. In addition, for cells exposed to a given x-ray dose, the number of transformed foci per dish is independent of the number of irradiated cells. These observations suggest that few, if any, of the transformed clones occur as a direct consequence of the x-ray exposure and challenge the hypothesis that transformed foci are the clonal products of occasional cells that have experienced an x-ray-induced mutational change. Rather, it appears that at least two steps are involved. We suggest that exposure to x-rays results in a change, for example, the induction or expression of some cell function, in many or all of the cells and that this change is transmitted to the progeny of the surviving cells; a consequence of this change is an enhanced probability of the occurrence of a second step, transformation, when these cells are maintained under conditions of confluence.
- OSTI ID:
- 6565148
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 77:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
ONCOGENIC TRANSFORMATIONS
RADIOINDUCTION
CELL CULTURES
CELL PROLIFERATION
CLONE CELLS
DELAYED RADIATION EFFECTS
DOSE-RESPONSE RELATIONSHIPS
GENE MUTATIONS
MICE
RADIATION DOSES
X RADIATION
ANIMALS
BIOLOGICAL EFFECTS
DOSES
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
MUTATIONS
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
550200 - Biochemistry