Immobilization of cellulolytic and hemicellulolytic enzymes on inorganic supports
Commercial cellulase preparations from Trichoderma viride and Aspergillus niger were immobilized on porous silica glass and ceramics such as alumina and titania with titanium tetrachloride (TiCl/sub 4/) and on their silanized derivatives with glutaraldehyde (GLUT). The amounts of the immobilized enzymes were in the range 10-50 mg/g carrier (dry) depending on the kind of carrier and immobilization method. Their activities toward carboxymethyl cellulose (CMC), xylan, aryl-..beta..-glucoside, and aryl-..beta..-xyloside were 3-53% of those of the native enzymes. The optimum pH of the enzymes shifted to the acidic side in most cases, whereas the optimum temperatures were nearly the same as those of native ones. The activity of immobilized enzyme preparations toward CMC did not change significantly during continuous operation over a period of 60 days. Finally, xylan was hydrolyzed with the immobilized enzymes, and the sugars formed were investigated. 22 reference.
- Research Organization:
- Forestry and Forest Products Research Institute, Ibaraki, Japan
- OSTI ID:
- 6564169
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 29:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CELLULASE
ENZYME ACTIVITY
IMMOBILIZED ENZYMES
ALUMINIUM OXIDES
ASPERGILLUS
CELLULOSE
ENZYMATIC HYDROLYSIS
OPTIMIZATION
PH VALUE
QUANTITY RATIO
SILICA
SUPPORTS
TITANIUM OXIDES
TRICHODERMA VIRIDE
ALUMINIUM COMPOUNDS
CARBOHYDRATES
CHALCOGENIDES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
MECHANICAL STRUCTURES
MINERALS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
OXIDE MINERALS
OXIDES
OXYGEN COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SILICON COMPOUNDS
SILICON OXIDES
SOLVOLYSIS
TITANIUM COMPOUNDS
TRANSITION ELEMENT COMPOUNDS
TRICHODERMA
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