GTP-binding peptide of beta-tubulin. Localization by direct photoaffinity labeling and comparison with nucleotide-binding proteins

Journal Article · Sat Oct 15 00:00:00 EDT 1988 · J. Biol. Chem.; (United States)

OSTI ID:6561751

Linse, K; Mandelkow, E M

The binding site of the guanine moiety of GTP on beta-tubulin was located within the peptide consisting of residues 63-77, AILVDLEPGTMDSVR. The result was obtained using direct photoaffinity labeling, peptide sequencing, and limited proteolysis. Peptides were identified by end-labeling with a monoclonal antibody against beta-tubulin whose epitope was located between 3 and 4 kDa from the C terminus. The sequence of the GTP-binding site is consistent with predictions from other GTP-binding proteins such as elongation factor Tu or ras p21.

• Research Organization: Max-Planck-Unit for Structural Molecular Biology, DESY, Hamburg (Germany, F.R.)
• OSTI ID: 6561751
• Journal Information: J. Biol. Chem.; (United States), Vol. 263:29
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NUCLEOTIDES
RECEPTORS
PEPTIDES
LABELLING
AMINO ACID SEQUENCE
BRAIN
CHYMOTRYPSIN
MONOCLONAL ANTIBODIES
PROTEOLYSIS
SWINE
TRACER TECHNIQUES
ANIMALS
ANTIBODIES
BODY
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
DECOMPOSITION
DOMESTIC ANIMALS
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
MAMMALS
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PROTEINS
SERINE PROTEINASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques