Properties of cellulase immobilized on agarose gel with spacer
Journal Article
·
· Biotechnol. Bioeng.; (United States)
Cellulase produced by fungus Trichoderma viride was immobilized on agarose beads (Sepharose 4B) activated by cyanogen bromide and also on activated agarose beads that contained spacer arm (activated Ch-Sepharose 4B and Affi-Gel 15). The CMCase activity retained by immobilized cellulase on activated Sepharose containing the spacer tended to be higher than that immobilized without spacer, although the extent of protein immobilization was lower. Also, the higher substrate specificity for cellulase immobilized on beads with spacer was obtained for cellobiose, acid-swollen cellulose, or cellulose powder. The hydrolysis product from their substrates was mainly glucose. 10 references.
- Research Organization:
- National Food Research Institute, Ibaraki, Japan
- OSTI ID:
- 6561637
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 28:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
CELLULASE
ENZYME ACTIVITY
IMMOBILIZED ENZYMES
AGAR
CELLOBIOSE
CHEMICAL ACTIVATION
ENZYMATIC HYDROLYSIS
GELS
GLUCOSE
PH VALUE
POWDERS
PRODUCTION
PROTEINS
SUBSTRATES
TRICHODERMA VIRIDE
ALDEHYDES
CARBOHYDRATES
CHEMICAL REACTIONS
COLLOIDS
DECOMPOSITION
DISACCHARIDES
DISPERSIONS
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
TRICHODERMA
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
550700 - Microbiology
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
59 BASIC BIOLOGICAL SCIENCES
CELLULASE
ENZYME ACTIVITY
IMMOBILIZED ENZYMES
AGAR
CELLOBIOSE
CHEMICAL ACTIVATION
ENZYMATIC HYDROLYSIS
GELS
GLUCOSE
PH VALUE
POWDERS
PRODUCTION
PROTEINS
SUBSTRATES
TRICHODERMA VIRIDE
ALDEHYDES
CARBOHYDRATES
CHEMICAL REACTIONS
COLLOIDS
DECOMPOSITION
DISACCHARIDES
DISPERSIONS
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
TRICHODERMA
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
550700 - Microbiology
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)