Comparative genomic hybridization reveals many new loci containing amplified genes in breast cancer

Conference · Fri Jan 01 00:00:00 EST 1993 · Cytometry (Baltimore); (United States)

OSTI ID:6558362

Kallioniemi, O P; Kallioniemi, A H; Rutovitz, D; Sudar, D; Chen, L C; Smith, H S; Gray, J W; Pinkel, D; Waldman, F M ^[1]

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1. Univ. of California, San Francisco (United States) GBCRI, San Francisco, CA (United States) MRC Genetics Unit, Edinburgh (United Kingdom)

The authors have developed a powerful new technique, comparative genomic hybridization (CGH), for molecular cytogenetic analysis of solid tumors. In CGH, differentially labeled total tumor DNA and normal reference DNA are allowed to compete for their binding sites in a normal metaphase spread. After immunofluorescent staining, the relative copy numbers of all regions in the tumor genome can be quantitiated with an image analysis system by measuring the intensity ratios of the two fluorochromes along the length of each chromosome. They used CGH to study gene amplification and other chromosomal changes in 16 breast cancer cell lines and 20 primary tumors. The loci undergoing amplification were highly variable from one sample to another and over 20 distinct sites were identified. Some sites correspond to locations of known oncogenes (e.g. erbb2 at 17q12 and myc at 8q24) whereas most of them (e.g. 6p22, 11p15, 17q22) are not previously known to contain amplified oncogenes. Frequent changes affecting larger chromosomal regions, such as duplications of 1q and deletions of 1p32-35, have also been found. CGH can dramatically facilitate identification of commonly altered chromosomal loci in cancer.

• DOE Contract Number: AC03-76SF00098
• OSTI ID: 6558362
• Report Number(s): CONF-9303114-; CODEN: CYTODQ; CNN: CA 45919; CA 47537
• Journal Information: Cytometry (Baltimore); (United States), Vol. 6; Conference: 16. congress of the International Society for Analytical Cytology, Colorado Springs, CO (United States), 21-26 Mar 1993; ISSN 0196-4763
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMAL ABERRATIONS
DETECTION
GENE AMPLIFICATION
MAMMARY GLANDS
NEOPLASMS
CHROMOSOMES
DNA HYBRIDIZATION
FLUORESCENCE
HUMAN CHROMOSOME 17
IMMUNOASSAY
ONCOGENES
BIOASSAY
BODY
DISEASES
GENES
GLANDS
HUMAN CHROMOSOMES
HYBRIDIZATION
LUMINESCENCE
MUTATIONS
ORGANS
550400* - Genetics