Ultraviolet radiation alters choline phospholipid metabolism in human keratinocytes
Ultraviolet radiation B (UVB-290-320 nm) induces inflammation and hyperproliferation in human epidermis. This response is associated with the recovery from irradiated skin of inflammatory mediators derived from membrane phospholipids. We have previously reported that UVB stimulates the production of such mediators by human keratinocytes (HK) in culture. In these studies we examined the effect of UVB on the metabolism of choline containing phospholipids in HK prelabeled with (/sup 3/H) choline. UVB (400-1600J/m2) stimulated a dose dependent release of (/sup 3/H) choline from HK within minutes of irradiation. Examination of media extracts by paper chromatography revealed that the released (/sup 3/H) choline was predominately in the form of glycerophosphorylcholine. Examination of label remaining in membranes of cells after irradiation by acid precipitation and HPLC revealed that the origin of the released (/sup 3/H) choline was the membrane phosphatidylcholine/lysophosphatidylcholine. These data support a concept of UVB stimulation of both a phospholipase A (1 or 2) and a lysophospholipase. These UVB induced alterations of HK membrane phospholipid metabolism likely have profound effects on UVB-induced inflammation and control of cell growth in human skin.
- Research Organization:
- Columbia Univ., NY (USA)
- OSTI ID:
- 6557902
- Journal Information:
- J. Invest. Dermatol.; (United States), Vol. 91:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CHOLINE
METABOLISM
EPIDERMIS
BIOLOGICAL RADIATION EFFECTS
ANIMAL CELLS
CELL MEMBRANES
CELL PROLIFERATION
DOSE-RESPONSE RELATIONSHIPS
KERATIN
LIPASE
LIQUID COLUMN CHROMATOGRAPHY
MAN
MEMBRANE PROTEINS
PHOSPHOLIPIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOXYLESTERASES
CELL CONSTITUENTS
CHROMATOGRAPHY
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
EPITHELIUM
ESTERASES
ESTERS
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIPOTROPIC FACTORS
MAMMALS
MEMBRANES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PRIMATES
PROTEINS
QUATERNARY COMPOUNDS
RADIATION EFFECTS
RADIATIONS
SCLEROPROTEINS
SEPARATION PROCESSES
SKIN
TISSUES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
550201 - Biochemistry- Tracer Techniques