Multiplex sequencing
This patent describes a method for sequencing a DNA specimen. It comprises: providing a set of at least two vectors, each vector of the set comprising a cloning site and including at least one tag sequence at a position within 50 bases of the cloning site, each tag sequence in each vector differing from each tag sequence of every other vector of the set, and each being incapable of hybridizing to the DNA specimen under stringent conditions, providing a first and a second DNA sequence from the DNA specimen. The first DNA sequence being different from the second DNA sequence, ligating the first DNA sequence into the cloning site of one of the vectors, and the second DNA sequence into the cloning side of the second of the vectors, thereby producing a plurality of hybrid vectors, providing a pool of the hybrid vectors, treating separate aliquots of the pool in a plurality of vessels to produce fragments, separating the fragments comprising tag sequences from each the vessel according to their size, hybridizing the separated fragments under stringent conditions with a first oligonucleotide probe able to hybridize specifically with one of the tag sequences, and detecting the pattern of hybridization wherein the pattern reflects the nucleotide sequence of the DNA specimen.
- Assignee:
- Harvard College, Cambridge, MA (USA)
- Patent Number(s):
- US 4942124; A
- Application Number:
- PPN: US s 7-228596
- OSTI ID:
- 6555627
- Resource Relation:
- Patent File Date: 4 Aug 1988
- Country of Publication:
- United States
- Language:
- English
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