Absence of induction of enhanced reactivation of herpes simplex virus in cells from xeroderma pigmentosum patients without skin cancer

Journal Article · Tue Nov 01 00:00:00 EST 1988 · Cancer Res.; (United States)

OSTI ID:6555381

Abrahams, P J; van der Kleij, A A; Schouten, R; van der Eb, A J

The time course of appearance of enhanced reactivation (ER) and enhanced mutagenesis (EM) of herpes simplex virus type 1 were studied in UV-irradiated stationary cultures of xeroderma pigmentosum (XP) fibroblasts. In some of the XP cells EM followed similar kinetics of appearance as ER. Maximal activities occurred when infection was delayed 1 or 2 days after cell treatment. However, in certain XP cells only induction of the EM response was observed, whereas ER was absent. Interestingly, the latter XP cells had been obtained from patients who had not yet developed skin cancer at the time they were described in the literature, whereas the former XP patients had already developed skin tumors. This suggests that the ER response may somehow be involved in the process of oncogenic transformation. Dose-response studies of ER in XP cells from tumor-bearing patients showed that ER is maximally induced with a UV dose of 40 Jm-2 given to the virus. Normal levels of ER were observed in 14 different normal human skin fibroblasts, indicating that the ER- phenotype does not occur in normal cells or at least more rarely than in XP cells.

• Research Organization: Sylvius Laboratories, Leiden (Netherlands)
• OSTI ID: 6555381
• Journal Information: Cancer Res.; (United States), Vol. 48:21
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HERPES SIMPLEX
VIABILITY
MUTATIONS
RADIOINDUCTION
CELL CULTURES
DNA REPAIR
DOSE-RESPONSE RELATIONSHIPS
FIBROBLASTS
GENETIC RADIATION EFFECTS
PHENOTYPE
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM
XP CELLS
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
INFECTIOUS DISEASES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SKIN DISEASES
SOMATIC CELLS
VIRAL DISEASES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture