Structure of plant cell walls: XIX. Isolation and characterization of wall polysaccharides from suspension-cultured Douglas fir cells
The partial purification and characterization of cell wall polysaccharides isolated from suspension-cultured Douglas fir (Pseudotsuga menziesii) cells are described. Extraction of isolated cell walls from 1.0 M LiCl solubilized pectic polysaccharides with glycosyl-linkage compositions similar to those of rhamnogalacturonans I and II, pectic polysaccharides isolated from walls of suspension-cultured sycamore cells. Treatment of LiCl-extracted Douglas fir walls with an endo-..cap alpha..-1,4-polygalacturonase released only small, additional amounts of pectic polysaccharide, which had a glycosyl-linkage composition similar to that of rhamnogalacturonan I. Xyloglucan oligosaccharides were released from the endo-..cap alpha..-1,4-polygalacturonase-treated walls by treatment with an endo-..beta..-1,4-glucanase. These oligosaccharides included hepta- and nonasaccharides similar or identical to those released from sycamore cell walls by the same enzyme, and structurally related octa- and decasaccharides similar to those isolated from various angiosperms. Finally, additional xyloglucan and small amounts of xylan were extracted from the endo-..beta..-1,4-glucanase-treated walls by 0.5 N NaOH. The xylan resembled that extracted by NaOH from dicot cell walls in that it contained 2,4- but not 3,4-linked xylosyl residues. In this study, a total of 15% of the cell wall was isolated as pectic material, 10% as xyloglucan, and less than 1% as xylan. The noncellulosic polysaccharides accounted for 25% of the cell walls, cellulose for 23%, protein for 34%, and ash for 5%, for a total of 88% of the cell wall.
- Research Organization:
- Univ. of Georgia, Athens, GA (United States)
- OSTI ID:
- 6550115
- Journal Information:
- Plant Physiol.; (United States), Vol. 83:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL WALL
MOLECULAR STRUCTURE
POLYSACCHARIDES
CHEMICAL COMPOSITION
FRACTIONATION
CELLULASE
FIRS
PLANT CELLS
CARBOHYDRATES
CELL CONSTITUENTS
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SEPARATION PROCESSES
TREES
550300* - Cytology