Enhancement of thermal response of normal and malignant tissues by Corynebacterium parvum. [Mice]
Further studies were carried out on the combined effects of Corynebacterium parvum and hyperthermia on animal tissues and cultured Chinese hamster ovary cells. Experimental animals were C3Hf/Sed mice derived from a defined flora mouse colony. Tumors were eighth-generation isotransplants of a spontaneous fibrosarcoma, FSa-II. Hyperthermia was given by immersing the mouse foot or culture flasks in the constant temperature water bath. Present experiments include thermal enhancement of C. parvum at different temperatures, effect of the agent on the kinetics of thermal resistance, and the mechanism of the thermal enhancement. The thermal enhancement by C. parvum was independent of temperature in a range between 42.5 and 46.5 degrees, and it increased with decreasing temperature. The analysis of the Arrhenius plot suggested a comparable activation energy for combined treatments and for heat alone between 42.5 and 46.5 degrees. The thermal resistance developed very rapidly in both normal and tumor tissues. Systemic administration of C. parvum failed to modify the kinetics of thermal resistance. Several experiments were attempted in order to disclose the mechanism. A single injection of C. parvum-induced macrophages failed to enhance thermal response of the mouse foot, while 3 daily injections of the macrophages enhanced the response, indicating that the enhancement by C. parvum is at least partly attributed to the C. parvum-induced macrophages. Whole-body irradiation of 6 Gy and/or administration of anti-mouse T-cell serum and histamine failed to inhibit the C. parvum enhancement of thermal response. No thermal enhancement was observed for Chinese hamster ovary cells treated at 43.0 degrees in vitro with C. parvum or thiomersalate, a preservative supplemented in C. parvum, although cytotoxic effect was shown at a high concentration of thiomersalate.
- Research Organization:
- Massachusetts General Hospital, Boston
- OSTI ID:
- 6539059
- Journal Information:
- Cancer Res.; (United States), Vol. 44:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EXPERIMENTAL NEOPLASMS
THERAPY
HYPERTHERMIA
RADIOSENSITIVITY EFFECTS
RETICULOENDOTHELIAL SYSTEM
BIOLOGICAL RADIATION EFFECTS
ANIMAL TISSUES
BACTERIAL DISEASES
BIOLOGICAL EFFECTS
CHO CELLS
FIBROSARCOMAS
IMMUNE REACTIONS
MACROPHAGES
MICE
TEMPERATURE EFFECTS
WHOLE-BODY IRRADIATION
ANIMAL CELLS
ANIMALS
BODY
BODY TEMPERATURE
CONNECTIVE TISSUE CELLS
DISEASES
EXTERNAL IRRADIATION
INFECTIOUS DISEASES
IRRADIATION
MAMMALS
NEOPLASMS
PHAGOCYTES
RADIATION EFFECTS
RODENTS
SARCOMAS
SOMATIC CELLS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals