U.S. Department of EnergyOffice of Scientific and Technical Information
Combined autoradiography and immunofluorescence for estimation of single cell activity by ammonium-oxidizing bacteria
Abstract
Immunofluorescence and /sup 14/CO/sub 2/ autoradiography were used for simultaneously enumerating and assaying the autotrophic activity of ammonium-oxidizing bacteria in seawater. Relative activity (/sup 14/CO/sub 2/ assimilation as measured by autoradiography) and abundance were measured in simulated in situ incubations at seven stations in the primary NO/sub 2//sup -/ maximum region of the Northeast Pacific Ocean. More than 10/sup 4/ cells liter/sup -1/ were present; relative activity often showed a peak near the surface and an increase in the NO/sub 2//sup -/ max region below the photic zone. The method permits assessment of individual cell activity; most cells at all depths were active in CO/sub 2/ assimilation, usually at low and quite variable levels. There were no differences in relative activity between samples incubated under simulated in situ conditions and in the dark. Relative activity was positively correlated with the abundance of ammonium-oxidizing bacteria, temperature, total dark CO/sub 2/ assimilation (as measured by liquid scintillation counting of replicate samples), and pheopigment concentration, and negatively correlated with oxygen concentration.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Washington, Seattle, WA (United States)
- OSTI Identifier:
- 6538646
- DOE Contract Number:
- AT06-76EV75026
- Resource Type:
- Journal Article
- Journal Name:
- Limnol. Oceanogr.; (United States)
- Additional Journal Information:
- Journal Volume: 29:2
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; BACTERIA; METABOLISM; SEAWATER; NITRIFICATION; AMMONIUM COMPOUNDS; AUTORADIOGRAPHY; CARBON 14 COMPOUNDS; CARBON DIOXIDE; NITROGEN DIOXIDE; TRACER TECHNIQUES; CARBON COMPOUNDS; CARBON OXIDES; CHALCOGENIDES; CHEMICAL REACTIONS; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MICROORGANISMS; NITROGEN COMPOUNDS; NITROGEN OXIDES; OXIDES; OXYGEN COMPOUNDS; WATER; 550701* - Microbiology- Tracer Techniques
Citation Formats
Ward, B B. Combined autoradiography and immunofluorescence for estimation of single cell activity by ammonium-oxidizing bacteria. United States: N. p., 1984.
Web. doi:10.4319/lo.1984.29.2.0402.
Ward, B B. Combined autoradiography and immunofluorescence for estimation of single cell activity by ammonium-oxidizing bacteria. United States. https://doi.org/10.4319/lo.1984.29.2.0402
Ward, B B. 1984.
"Combined autoradiography and immunofluorescence for estimation of single cell activity by ammonium-oxidizing bacteria". United States. https://doi.org/10.4319/lo.1984.29.2.0402.
@article{osti_6538646,
title = {Combined autoradiography and immunofluorescence for estimation of single cell activity by ammonium-oxidizing bacteria},
author = {Ward, B B},
abstractNote = {Immunofluorescence and /sup 14/CO/sub 2/ autoradiography were used for simultaneously enumerating and assaying the autotrophic activity of ammonium-oxidizing bacteria in seawater. Relative activity (/sup 14/CO/sub 2/ assimilation as measured by autoradiography) and abundance were measured in simulated in situ incubations at seven stations in the primary NO/sub 2//sup -/ maximum region of the Northeast Pacific Ocean. More than 10/sup 4/ cells liter/sup -1/ were present; relative activity often showed a peak near the surface and an increase in the NO/sub 2//sup -/ max region below the photic zone. The method permits assessment of individual cell activity; most cells at all depths were active in CO/sub 2/ assimilation, usually at low and quite variable levels. There were no differences in relative activity between samples incubated under simulated in situ conditions and in the dark. Relative activity was positively correlated with the abundance of ammonium-oxidizing bacteria, temperature, total dark CO/sub 2/ assimilation (as measured by liquid scintillation counting of replicate samples), and pheopigment concentration, and negatively correlated with oxygen concentration.},
doi = {10.4319/lo.1984.29.2.0402},
url = {https://www.osti.gov/biblio/6538646},
journal = {Limnol. Oceanogr.; (United States)},
number = ,
volume = 29:2,
place = {United States},
year = {Thu Mar 01 00:00:00 EST 1984},
month = {Thu Mar 01 00:00:00 EST 1984}
}
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