Mutagenesis of dimeric plasmids by the transposon. gamma. delta. (Tn1000)
Journal Article
·
· Journal of Bacteriology; (USA)
OSTI ID:6534210
- Univ. of Connecticut, Storrs (USA)
The Escherichia coli F factor mediates conjugal transfer of a plasmid such as pBR322 primarily by replicative transposition of transposon {gamma}{delta} (Tn1000) from F to that plasmid to form a cointegrate intermediate. Although resolution of this cointegrate always yields a plasmid containing a single {gamma}{delta} insertion, the occasional recovery of transposon-free plasmids after connuugal transfer has led to alternative hypotheses for F mobilization. The authors show here that {gamma}{delta}-free plasmids are found after F-mediated conjugal transfer only when the donor plasmid is a dimer and the recipient is Rec{sup +}.
- DOE Contract Number:
- FG02-89ER60862
- OSTI ID:
- 6534210
- Journal Information:
- Journal of Bacteriology; (USA), Vol. 172:5; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
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