O-linked glycosylation of retroviral envelope gene products

Journal Article · Tue Mar 01 00:00:00 EST 1988 · Journal of Virology; (USA)

OSTI ID:6533465

Pinter, A; Honnen, W J ^[1]

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1. Public Health Research Institute of the City of New York Inc., NY (USA)

Treatment of ({sup 3}H)glucosamine-labeled Friend mink cell focus-forming virus (FrMCF) gp70 with excess peptide:N-glycanase F (PNGase F) resulted in removal of the expected seven N-linked oligosaccharide chains; however, approximately 10% of the glucosamine label was retained in the resulting 49,000-M{sub r} (49K) product. For ({sup 3}H)mannose-labeled gp70, similar treatment led to removal of all the carbohydrate label from the protein. Prior digestion of the PNGase F-treated gp70 with neuraminidase resulted in an addition size shift, and treatment with O-glycanase led to the removal of almost all of the PNGase F-resistant sugars. These results indicate that gp70 possesses sialic acid-containing O-linked oligosaccharides. Analysis of intracellular env precursors demonstrated that O-linked sugars were present in gPr90{sup env}, the polyprotein intermediate which contains complex sugars, but not in the primary translation product, gPr80{sup env}, and proteolytic digestion studies allowed localization of the O-linked carbohydrates to a 10K region near the center of the gp70 molecule. similar substituents were detected on the gp70s of ecotropic and xenotropic murine leukemia viruses and two subgroups of feline leukemia virus, indicting that O-linked glycosylation is a conserved feature of retroviral env proteins.

• OSTI ID: 6533465
• Journal Information: Journal of Virology; (USA), Vol. 62:3; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
O-GLYCOSYL HYDROLASES
BIOCHEMISTRY
OLIGOSACCHARIDES
ENZYMATIC HYDROLYSIS
CYSTEINE
ELECTROPHORESIS
GLUCOSAMINE
MANNOSE
PRECIPITATION
RADIOIMMUNOLOGY
SULFUR 35
TRITIUM COMPOUNDS
VIRUSES
ALDEHYDES
AMINES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ENZYMES
EVEN-ODD NUCLEI
GLYCOSYL HYDROLASES
HEXOSAMINES
HEXOSES
HYDROGEN COMPOUNDS
HYDROLASES
HYDROLYSIS
IMMUNOLOGY
ISOTOPES
LIGHT NUCLEI
LYSIS
MICROORGANISMS
MONOSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
RADIOISOTOPES
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
SULFUR ISOTOPES
THIOLS
550201* - Biochemistry- Tracer Techniques