Role of cytochrome P sub 450 in the control of the production of erythropoietin

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90225-G· OSTI ID:6533251

Fandrey, J; Seydel, F P; Siegers, C P; Jelkmann, W ^[1]

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1. Medical Univ. of Luebeck (West Germany)

Effects of agents affecting cytochrome P{sub 450} were studied on the production of erythropoietin (Epo) in cultures of the human hepatoma cell line HepG2. Epo was measured by radioimmunoassay of the culture media after 24 h of incubation. The addition of phenobarbital or 3-methylcholanthrene, which induce cytochrome P{sub 450}, significantly enhanced the formation of Epo. Likewise, the thyroid hormones T{sub 3} and T{sub 4} stimulated the rate of the production of Epo. On the other hand, the formation of Epo was lowered following the addition of diethyl-dithiocarbamate or cysteamine chloride, which inhibit cytochrome P{sub 450}. These findings support the idea that O{sub 2} sensitive hemoproteins of the microsomal mixed-functional oxidases play a role in the control of the synthesis of Epo.

• OSTI ID: 6533251
• Journal Information: Life Sciences; (USA), Vol. 47:2; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
3-METHYLCHOLANTHRENE
BIOLOGICAL EFFECTS
ERYTHROPOIETIN
BIOSYNTHESIS
MIXED-FUNCTION OXIDASES
BIOLOGICAL FUNCTIONS
ENZYME INDUCTION
ENZYME INHIBITORS
HEPATOMAS
MAN
MICROSOMES
PHENOBARBITAL
RADIOIMMUNOASSAY
THYROID HORMONES
TUMOR CELLS
ANESTHETICS
ANIMAL CELLS
ANIMALS
ANTICONVULSANTS
AROMATICS
AZINES
BARBITURATES
BIOASSAY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIAGNOSTIC TECHNIQUES
DISEASES
DRUGS
ENZYMES
FUNCTIONS
GENE REGULATION
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROCARBONS
HYPNOTICS AND SEDATIVES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MITOGENS
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
OXYGENASES
PEPTIDE HORMONES
POLYCYCLIC AROMATIC HYDROCARBONS
PRIMATES
PROTEINS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RIBOSOMES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques