( sup 3 H) D-serine labels strychnine-insensitive glycine recognition sites of rat central nervous system

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90100-6· OSTI ID:6532917

Danysz, W; Fadda, E; Wroblewski, J T; Costa, E ^[1]

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1. Georgetown Univ., Washington, DC (USA)

In the central nervous system, glycine binds to two recognition sites; one of them (G{sub 2}), associated with the glutamate receptor, is insensitive to strychnine. Strychnine-insensitive sites were predominant in the forebrain areas and bound D-serine and D-alanine better than the respective L stereoisomers. ({sup 3}H) D-serine was a more selective radioligand than ({sup 3}H)glycine for the strychnine-insensitive sites. In the forebain, the binding of both ligands was inhibited by the putative G{sub 2} receptor antagonists, 7-chlorokynurenate and 3-amino-1-hydroxy-2-pyrrolidone, while in pons and in spinal cord only the latter drug was effective. This may indicate the heterogeneity of strychnine-insensitive glycine recognition sites.

• OSTI ID: 6532917
• Journal Information: Life Sciences; (USA), Vol. 46:3, Issue 3; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUTAMIC ACID
RECEPTORS
GLYCINE
BIOCHEMICAL REACTION KINETICS
STRYCHNINE
BIOLOGICAL EFFECTS
CENTRAL NERVOUS SYSTEM
ELECTROPHYSIOLOGY
LIGANDS
RATS
SERINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALOIDS
AMINO ACIDS
ANIMALS
AROMATICS
AZAARENES
AZOLES
CARBOXYLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
INDOLES
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYSIOLOGY
PROTEINS
PYRROLES
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques