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Title: Arachidonate metabolism increases as rat alveolar type II cells differentiate in vitro

Abstract

Rat type II alveolar epithelial cells are known to undergo morphological and functional changes when maintained in culture for several days. Having previously demonstrated that these cells can deacylate free arachidonic acid (AA) and metabolize it to products of the cyclooxygenase pathway, the present study was undertaken to determine whether in vitro differentiation was accompanied by alterations in the availability and metabolism of AA. We assessed the constitutive and ionophore A23187-induced deacylation and metabolism of endogenous AA, as well as the metabolism of exogenously supplied AA, in primary cultures of rat type II cells at days 2, 4, and 7 after isolation. Levels of free endogenous AA were increased at day 4, whereas eicosanoid synthesis, predominantly prostaglandin E2 and prostacyclin, increased markedly only at day 7. A similar time course of augmentation of prostanoid release was seen in response to exogenous AA. Type II cells cultured on fibronectin, intended to hasten cell flattening and spreading, demonstrated accelerated increases in available free AA in response to A23187; cells cultured on basement membrane derived from Engelbreth-Holm-Swarm mouse sarcoma, known to maintain the type II phenotype, exhibited diminished levels of available free AA. From these findings, we conclude that alterations in arachidonate metabolismmore » are linked to alterations in cellular phenotype. The potentiation of eicosanoid synthesis accompanying in vitro differentiation suggests a possible role for the alveolar epithelium in the modulation of inflammation and fibrosis in the distal lung.« less

Authors:
; ; ; ;  [1]
  1. Univ. of Michigan, Ann Arbor (USA)
Publication Date:
OSTI Identifier:
6529866
Resource Type:
Journal Article
Journal Name:
American Journal of Physiology; (USA)
Additional Journal Information:
Journal Volume: 259:2 Pt 1; Journal ID: ISSN 0002-9513
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANIMAL CELLS; CELL DIFFERENTIATION; ARACHIDONIC ACID; METABOLISM; EICOSANOIC ACID; BIOSYNTHESIS; LUNGS; FIBROSIS; CARBON ISOTOPES; CELL CULTURES; EPITHELIUM; FLUORESCENCE; ISOTOPE DILUTION; LIQUID COLUMN CHROMATOGRAPHY; MORPHOLOGICAL CHANGES; PHENOTYPE; PROSTAGLANDINS; RATS; ANIMAL TISSUES; ANIMALS; BODY; CARBOXYLIC ACIDS; CHROMATOGRAPHY; ISOTOPE APPLICATIONS; ISOTOPES; LUMINESCENCE; MAMMALS; MONOCARBOXYLIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PATHOLOGICAL CHANGES; RESPIRATORY SYSTEM; RODENTS; SEPARATION PROCESSES; SYNTHESIS; TISSUES; TRACER TECHNIQUES; VERTEBRATES; 550501* - Metabolism- Tracer Techniques; 550901 - Pathology- Tracer Techniques

Citation Formats

Lipchik, R J, Chauncey, J B, Paine, R, Simon, R H, and Peters-Golden, M. Arachidonate metabolism increases as rat alveolar type II cells differentiate in vitro. United States: N. p., 1990. Web.
Lipchik, R J, Chauncey, J B, Paine, R, Simon, R H, & Peters-Golden, M. Arachidonate metabolism increases as rat alveolar type II cells differentiate in vitro. United States.
Lipchik, R J, Chauncey, J B, Paine, R, Simon, R H, and Peters-Golden, M. 1990. "Arachidonate metabolism increases as rat alveolar type II cells differentiate in vitro". United States.
@article{osti_6529866,
title = {Arachidonate metabolism increases as rat alveolar type II cells differentiate in vitro},
author = {Lipchik, R J and Chauncey, J B and Paine, R and Simon, R H and Peters-Golden, M},
abstractNote = {Rat type II alveolar epithelial cells are known to undergo morphological and functional changes when maintained in culture for several days. Having previously demonstrated that these cells can deacylate free arachidonic acid (AA) and metabolize it to products of the cyclooxygenase pathway, the present study was undertaken to determine whether in vitro differentiation was accompanied by alterations in the availability and metabolism of AA. We assessed the constitutive and ionophore A23187-induced deacylation and metabolism of endogenous AA, as well as the metabolism of exogenously supplied AA, in primary cultures of rat type II cells at days 2, 4, and 7 after isolation. Levels of free endogenous AA were increased at day 4, whereas eicosanoid synthesis, predominantly prostaglandin E2 and prostacyclin, increased markedly only at day 7. A similar time course of augmentation of prostanoid release was seen in response to exogenous AA. Type II cells cultured on fibronectin, intended to hasten cell flattening and spreading, demonstrated accelerated increases in available free AA in response to A23187; cells cultured on basement membrane derived from Engelbreth-Holm-Swarm mouse sarcoma, known to maintain the type II phenotype, exhibited diminished levels of available free AA. From these findings, we conclude that alterations in arachidonate metabolism are linked to alterations in cellular phenotype. The potentiation of eicosanoid synthesis accompanying in vitro differentiation suggests a possible role for the alveolar epithelium in the modulation of inflammation and fibrosis in the distal lung.},
doi = {},
url = {https://www.osti.gov/biblio/6529866}, journal = {American Journal of Physiology; (USA)},
issn = {0002-9513},
number = ,
volume = 259:2 Pt 1,
place = {United States},
year = {Wed Aug 01 00:00:00 EDT 1990},
month = {Wed Aug 01 00:00:00 EDT 1990}
}