Whole-body autoradiographic distribution of exogenously administered renal renin in rats

Journal Article · Fri May 01 00:00:00 EDT 1987 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/35.5.3549890· OSTI ID:6529200

Kim, S; Iwao, H; Nakamura, N; Ikemoto, F; Yamamoto, K

We studied, by whole-body autoradiography, the distribution of exogenously administered renal renin in rat. Rat renal renin was completely purified and labeled with /sup 125/I ((/sup 125/I)-renin) and was then injected into the tail veins of conscious rats at a dose of 30 microCi, 430 ng. After various intervals, rats were killed by an overdose of ether, the whole body rapidly frozen in acetone-dry ice, and autoradiography performed on sagittal whole-body sections. To remove breakdown products ((/sup 125/I)-tyrosine and free /sup 125/I) from (/sup 125/I)-renin, sections were treated with perchloric acid solution. The main accumulation of (/sup 125/I)-renin acid-insoluble radioactivity was observed in liver and renal cortex. The accumulation in these organs was already evident 2 min after the injection, reached a maximum level by 15 min, then gradually decreased. A small amount of (/sup 125/I)-renin was also evident in spleen, bone marrow, and adrenal gland. Thirty min after the injection, radioactivity began to appear in the thyroid gland, stomach, and small intestine, but disappeared with acid treatment, except in the thyroid. Radioactivity was negligible in other organs including brain, submaxillary gland, lung, heart, and testis. These autoradiographs clearly demonstrate that exogenously administered renal renin is distributed mainly in the liver and renal cortex.

• Research Organization: Osaka City Univ. Medical School, Japan
• OSTI ID: 6529200
• Journal Information: J. Histochem. Cytochem.; (United States), Vol. 5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BODY
AUTORADIOGRAPHY
IODINE 125
TISSUE DISTRIBUTION
RENIN
ADRENAL GLANDS
BONE MARROW
KIDNEYS
LIVER
PHARMACOLOGY
RATS
SMALL INTESTINE
SPLEEN
STOMACH
THYROID
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
ENZYMES
GASTROINTESTINAL TRACT
GLANDS
HEMATOPOIETIC SYSTEM
HYDROLASES
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE ISOTOPES
ISOTOPES
MAMMALS
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HYDROLASES
RADIOISOTOPES
RODENTS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques