A field evaluation of cytochrome P4501A as a biomarker of contaminant exposure in three species of flatfish
- National Oceanic and Atmospheric Administration, Seattle, WA (United States)
- Univ. of Bergen (Norway). Lab. of Marine Molecular Biology
A study was conducted over the course of a year to determine the induction of hepatic cytochrome P4501A (CYP1A) in three species of benthic fish collected from a contaminated site compared to fish sampled from a less-contaminated site. Juvenile fish were used to minimize effects of reproductive status and migration. CYP1A was determined by two catalytic assays [aryl hydrocarbon hydroxylase (AHH) and ethoxyresorufin O-deethylase (EROD)] and by an immunoassay (ELISA) utilizing polyclonal antibodies raised against purified CYP1A from cod. AHH activities were measured by a standard method (AHH[sub std]) and by two variations of the standard method. All three primary CYP1A measures (AHH[sub std], EROD, and ELISA) showed consistent between-site differences, indicating that induction of CYP1A can be a reliable biomarker of contaminant exposure in fish if appropriate biological variables are controlled for in field studies. Multiple ANOVA demonstrated that the AHH[sub std] and ELISA data showed less variability due to species or temporal differences, and less unexplained variability, compared to the data from the EROD assay or either variation of the AHH assay. For all measures, variability associated with site differences far outweighed species or temporal variability. Immunoassay, while less sensitive than the AHH[sub std] assay, is nonetheless recommended to be used in conjunction with catalytic assays because of the potential for samples to lose catalytic activity if not handled properly. The current results suggest that the lower noncontaminant-related variability of AHH[sub std] makes this CYP1A measure potentially more useful for monitoring programs in which analysis of trends is a primary goal.
- OSTI ID:
- 6527048
- Journal Information:
- Environmental Toxicology and Chemistry; (United States), Vol. 14:1; ISSN 0730-7268
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
FISHES
SENSITIVITY
OXIDOREDUCTASES
ENZYME INDUCTION
POLLUTANTS
TOXICITY
BENTHOS
BIOLOGICAL MARKERS
ENZYME ACTIVITY
METABOLIC ACTIVATION
WATER POLLUTION
ANIMALS
AQUATIC ORGANISMS
ENZYMES
GENE REGULATION
ORGANIC COMPOUNDS
POLLUTION
PROTEINS
VERTEBRATES
540320* - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)
560300 - Chemicals Metabolism & Toxicology