Method for producing intact plants containing foreign DNA
An in vivo method is described for transforming and regenerating whole dicotyledenous plants comprising: infecting a dicotyledenous plant (P) with Rhizobiaceae bacteria containing virulence functions; a first plasmid containing T-DNA terminal sequences flanking oncogenic factors capable of inducing a shoot-bearing shooty tumor on plant (P), and a second plasmid containing T-DNA terminal sequences flanking heterologous transfer DNA capable of being integrated into nuclear DNA of plant (P) cells wherein the second plasmid does not contain any oncogenic factors; maintaining the infected dicotyledenous plant (P) until shoot-bearing shooty tumor develops on the dicotyledenous plant (P); selecting those shoots, or progeny wherein the progeny are selected from the group consisting of seeds or tubers, that contain transformed cells having heterologous transfer DNA but not any tumorous DNA integrated into their genomes; and utilizing the selected shoots, or progeny to produce whole plants that contain cells having heterologous transfer DNA integrated into their genomes.
- Assignee:
- Atlantic Richfield Co., Los Angeles, CA
- Patent Number(s):
- US 4658082
- OSTI ID:
- 6525661
- Resource Relation:
- Patent File Date: Filed date 25 Jul 1984
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
PLANTS
DNA SEQUENCING
GENETIC ENGINEERING
BACTERIA
DNA
GENES
IN VIVO
NEOPLASMS
ONCOGENIC TRANSFORMATIONS
PLANT GROWTH
PLASMIDS
PROGENY
SEEDS
TUBERS
VIRULENCE
CELL CONSTITUENTS
CELL TRANSFORMATIONS
DISEASES
GROWTH
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
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