Increased activity of chondroitin sulfate-synthesizing enzymes during proliferation of arterial smooth muscle cells
Cultured arterial smooth muscle cells incorporate (/sup 35/S)sulfate into the extracellular chondroitin sulfate/dermatan sulfate containing proteoglycans at a higher rate in the phase of logarithmic growth than do non-dividing cells. The cell growth-dependent decrease in /sup 35/S incorporation with increasing cell density is accompanied by a decrease in the activity of chondroitin sulfate-synthesizing enzymes. The specific activity of xylosyl transferase, N-acetylgalactosaminyl transferase I and chondroitin sulfotransferase declines as the cells proceed from low to high densities. The corresponding correlation coefficients are 0.86, 0.91 and 0.89. The ratio of C-60H/C-40H sulfation of chondroitin shows a cell proliferation-dependent decrease indicating an inverse correlation of chondroitin 6-sulfotransferase and chondroitin 4-sulfotransferase activity. The observed changes in the expression of enzyme activities are thought to have some implications in the pathogenesis of arteriosclerosis, the initial stages of which are characterized by proliferation of arterial smooth muscle cells.
- Research Organization:
- Univ. of Muenster, West Germany
- OSTI ID:
- 6525365
- Journal Information:
- Exp. Cell Res.; (United States), Vol. 167:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ARTERIOSCLEROSIS
PATHOGENESIS
MUSCLES
ENZYME ACTIVITY
SULFUR 35
UPTAKE
ANIMAL CELLS
CELL PROLIFERATION
CHONDROITIN
GLYCOSYL TRANSFERASES
SULFATES
AMINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARDIOVASCULAR DISEASES
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
EVEN-ODD NUCLEI
ISOTOPES
LIGHT NUCLEI
MUCOPOLYSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
RADIOISOTOPES
SACCHARIDES
SULFUR COMPOUNDS
SULFUR ISOTOPES
TRANSFERASES
VASCULAR DISEASES
550201* - Biochemistry- Tracer Techniques