Visualization of viral clearance in the living animal
The early events in viral dissemination via the bloodstream were identified by monitoring the fate of /sup 123/I-radiolabeled reovirus after it was injected intravenously in rats. Continuous scintillation camera imaging showed that reovirus serotypes 1 and 3 were cleared from the circulation in less than 10 minutes by specific and distinct target organs. Reovirus serotype 1 accumulated predominantly in the lungs and the liver, whereas serotype 3 accumulated in the liver and the spleen with very little virus uptake by the lungs. Incubation of reovirus serotype 1 with a monoclonal antibody directed against the viral hemagglutinin before injection totally inhibited the clearance of the virus by the lungs. Similar results were obtained when viruses biolabeled with /sup 35/S were used. These results demonstrate that viruses can be rapidly transported through the bloodstream to specific target organs and that the localization of the viruses depends on the interaction between specific viral surface components and the target organ.
- Research Organization:
- Joslin Diabetes Center, Boston, MA
- OSTI ID:
- 6522669
- Journal Information:
- Science (Washington, D.C.); (United States), Vol. 4800
- Country of Publication:
- United States
- Language:
- English
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LIVER
SCINTISCANNING
LUNGS
VIRUSES
LUNG CLEARANCE
MEMBRANE TRANSPORT
BIOLOGICAL LOCALIZATION
INTRAVENOUS INJECTION
IODINE 123
MONOCLONAL ANTIBODIES
SPLEEN
SULFUR 35
TISSUE DISTRIBUTION
TRACER TECHNIQUES
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CLEARANCE
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
EXCRETION
GLANDS
HOURS LIVING RADIOISOTOPES
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PARASITES
RADIOISOTOPE SCANNING
RADIOISOTOPES
RESPIRATORY SYSTEM
SULFUR ISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics