Influence of immobilization on the stability of pTG201 recombinant plasmid in some strains of Escherichia coli
The stability of pTG201 plasmid was examined by continuous culture in three genetically different Escherichia coli hosts. Two types of experiment were carried out, one with free cells and one with immobilized cells. When cells were cultivated in free continuous culture in the absence of antibiotic selection, the plasmid was maintained with various degree of stability in the three host organisms. By contrast, in continuous culture with immobilized cells, plasmid pTG201 was stably maintained in the three strains. The authors showed that the increase in pTG201 stability in immobilized cells is due neither to plasmid transfer between immobilized cells nor to an increase of the plasmid copy number of immobilized cells. They also showed that plasmid-free cells, when coimmobilized and grown in competition with plasmid-containing cells, cannot overrun the culture.
- Research Organization:
- Universite de Technologie de Compiegne, France
- OSTI ID:
- 6521087
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 53:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Simple and Rapid Site-Specific Integration of Multiple Heterologous DNAs into the Escherichia coli Chromosome
Mobilization of plasmid pHSV106 from Escherichia coli HB101 a laboratory-scale waste treatment facility