Ethanol alters vasoactive intestinal peptide-induced steroid release from immature rat ovaries in vitro

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90101-V· OSTI ID:6518897

Dees, W L; Hiney, J K; Fuentes, F; Forrest, D W ^[1]

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1. Texas A M Univ., College Station (USA)

The present study was conducted to examine the acute effects of ethanol (ETOH) on basal and VIP-induced release of testosterone (T) and estradiol (E{sub 2}) from immature ovaries in vitro. Ovaries were collected from anestrus (A) and both naturally occurring and pregnant mare's serum gonadotropin (PMSG)-induced early proestrus (EP) animals. The ovaries were incubated in wither media alone, media plus 1 {mu}M VIP, media plus ETOH in doses ranging from 25 to 100 mM, or media plus each dose of ETOH containing VIP. The present results demonstrate that ETOH did not affect either basal or VIP-induced steroid release from ovaries collected from A animals. Likewise, the ETOH did not alter basal steroid secretion from EP animals; however, the drug significantly reduced the VIP-stimulated release of both T and E, from EP ovaries. Thus, these data demonstrate for the first time that ETOH is capable of altering prepubertal ovarian responsiveness to VIP, a peptide known to be involved in the developmental regulation of ovarian function.

• OSTI ID: 6518897
• Journal Information: Life Sciences; (USA), Vol. 46:3; ISSN 0024-3205
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ESTRADIOL
SECRETION
ETHANOL
BIOLOGICAL EFFECTS
OVARIES
BIOLOGICAL FUNCTIONS
TESTOSTERONE
IN VITRO
METABOLISM
PREGNANCY
RATS
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ANDROSTANES
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FUNCTIONS
GONADS
HORMONES
HYDROXY COMPOUNDS
KETONES
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ORGANS
RODENTS
STEROID HORMONES
STEROIDS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology