Autoradiographic localization of beta-adrenoreceptors in rat uterus

Journal Article · Thu Dec 01 00:00:00 EST 1988 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/36.12.2848069· OSTI ID:6518049

Tolszczuk, M; Pelletier, G

The inhibitory effects of catecholamines on uterine smooth muscle are known to be mediated through beta-adrenergic receptors. To investigate further the distribution of these receptors in the rat uterus, we utilized in vitro autoradiography using ( SVI)-cyanopindolol (CYP), a specific beta-receptor ligand that has equal activity for both beta 1- and beta 2-receptor subtypes. The specificity of the labeling and the characterization of receptor subtypes in different cell types were achieved by displacement of radioligand with increasing concentrations of zinterol, a beta-adrenergic agonist with preferential affinity for the beta 2-adrenoreceptor subtype, and practolol, a beta-adrenergic antagonist that binds preferentially to the beta 1-subtype. Quantitative estimation of ligand binding was performed by densitometry. It was shown that the vast majority of beta-adrenoreceptors were of the beta 2-subtype and were found in high concentration not only in the myometrium but also in the endometrial and serosal epithelia. Specific labeling was also observed in glandular elements. These results suggest that beta-adrenoreceptors might be involved in different functions in the uterus.

• Research Organization: Le Centre Hospitalier de l'Universite Laval, Ste-Foy, Quebec (Canada)
• OSTI ID: 6518049
• Journal Information: J. Histochem. Cytochem.; (United States), Vol. 36:12
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RECEPTORS
BIOLOGICAL LOCALIZATION
SYMPATHOMIMETICS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
CATECHOLAMINES
EPITHELIUM
IODINE 125
LABELLING
LIGANDS
RATS
TISSUE DISTRIBUTION
UTERUS
AMINES
ANIMAL TISSUES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FEMALE GENITALS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques