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Title: Lipoprotein lipase activity and chylomicron clearance in rats fed a high fat diet

Abstract

The relationships of tissue and plasma lipoprotein lipase (LPL) activities to tissue uptake and plasma clearance of UC-labeled chylomicron-triglyceride ( UC-CM-TG) were studied in female rats fed isoenergetic and isonitrogenous control (12% kJ from fat) or high fat diets (72% kJ from fat) for 8 wk. Animals fed the high-fat diet had higher levels of fasting plasma triglycerides and lower LPL activities in heart, renal adipose tissue and post-heparin plasma. Changes in LPL activities of skeletal muscles varied among muscles with higher values in the soleus and plantaris (32-61%) and no differences in the gastrocnemius. The lower LPL activity in renal adipose tissue was associated with lower uptake of fatty acids from UC-CM-TG by adipose. Fatty-acid uptake from labeled TG was not associated with tissue LPL activity in other tissues. Clearance of UC-CM-TG from plasma and the half-lives of UC-CM-TG were similar in both dietary groups. These data indicate that tissue and plasma LPL activities are not a direct index of uptake of fatty acids by tissues or clearance of chylomicron triglycerides.

Authors:
;
Publication Date:
Research Org.:
Univ. of Illinois, Urbana (USA)
OSTI Identifier:
6515280
Resource Type:
Journal Article
Journal Name:
J. Nutr.; (United States)
Additional Journal Information:
Journal Volume: 118:11
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; LIPASE; ENZYME ACTIVITY; TRIGLYCERIDES; BLOOD-PLASMA CLEARANCE; BIOLOGICAL HALF-LIFE; CARBON 14 COMPOUNDS; CARBOXYLIC ACIDS; CHYLOMICRONS; DIET; RATS; TISSUE DISTRIBUTION; TRACER TECHNIQUES; ANIMALS; CARBOXYLESTERASES; CLEARANCE; DISTRIBUTION; ENZYMES; ESTERASES; ESTERS; HYDROLASES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LIPIDS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; RODENTS; VERTEBRATES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Brown, C M, and Layman, D K. Lipoprotein lipase activity and chylomicron clearance in rats fed a high fat diet. United States: N. p., 1988. Web.
Brown, C M, & Layman, D K. Lipoprotein lipase activity and chylomicron clearance in rats fed a high fat diet. United States.
Brown, C M, and Layman, D K. 1988. "Lipoprotein lipase activity and chylomicron clearance in rats fed a high fat diet". United States.
@article{osti_6515280,
title = {Lipoprotein lipase activity and chylomicron clearance in rats fed a high fat diet},
author = {Brown, C M and Layman, D K},
abstractNote = {The relationships of tissue and plasma lipoprotein lipase (LPL) activities to tissue uptake and plasma clearance of UC-labeled chylomicron-triglyceride ( UC-CM-TG) were studied in female rats fed isoenergetic and isonitrogenous control (12% kJ from fat) or high fat diets (72% kJ from fat) for 8 wk. Animals fed the high-fat diet had higher levels of fasting plasma triglycerides and lower LPL activities in heart, renal adipose tissue and post-heparin plasma. Changes in LPL activities of skeletal muscles varied among muscles with higher values in the soleus and plantaris (32-61%) and no differences in the gastrocnemius. The lower LPL activity in renal adipose tissue was associated with lower uptake of fatty acids from UC-CM-TG by adipose. Fatty-acid uptake from labeled TG was not associated with tissue LPL activity in other tissues. Clearance of UC-CM-TG from plasma and the half-lives of UC-CM-TG were similar in both dietary groups. These data indicate that tissue and plasma LPL activities are not a direct index of uptake of fatty acids by tissues or clearance of chylomicron triglycerides.},
doi = {},
url = {https://www.osti.gov/biblio/6515280}, journal = {J. Nutr.; (United States)},
number = ,
volume = 118:11,
place = {United States},
year = {Tue Nov 01 00:00:00 EST 1988},
month = {Tue Nov 01 00:00:00 EST 1988}
}