Radiation effects on bovine taste bud membranes
In order to investigate the mechanisms of radiation-induced taste loss, the effects of radiation on preparations of enriched bovine taste bud membranes were studied. Taste buds containing circumvallate papilae, and surrounding control epithelial tissues devoid of taste buds, were obtained from steers and given radiation doses of 0-7000 cGy (rad). Tissue fractions were isolated into membrane-enriched and heterogeneous components using differential and sucrose gradient centrifugation of tissue homogenates. The yield of membranes, as measured by protein content in the buoyant membrane-enriched fractions, was reduced in quantity with increasing radiation dose. The relation between radiation dose and membrane quantity in membrane-enriched fractions could be fit by a simple exponential model with taste bud-derived membranes twice as radiosensitive as membranes from control epithelial tissue. Binding of sucrose, sodium, and acetate and fluoride stimulation of adenylate cyclase were nearly identical in both irradiated and nonirradiated intact membranes. Radiation had no effect on fractions of heterogeneous components. While it is not clear what changes are occurring in enriched taste cell membranes, damage to membranes may play an important role in the taste loss observed in patients following radiotherapy.
- Research Organization:
- Georgetown Univ. Medical Center, Washington, DC
- OSTI ID:
- 6514689
- Journal Information:
- Radiat. Res.; (United States), Vol. 92:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
RADIOTHERAPY
SIDE EFFECTS
TASTE BUDS
BIOLOGICAL RADIATION EFFECTS
CATTLE
CELL MEMBRANES
FLAVOR
PROTEINS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CELL CONSTITUENTS
DOMESTIC ANIMALS
MAMMALS
MEDICINE
MEMBRANES
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANOLEPTIC PROPERTIES
ORGANS
RADIATION EFFECTS
RADIOLOGY
RUMINANTS
SENSE ORGANS
THERAPY
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture