Quantitation of vitamin K in human milk
- Univ. of Arizona, Tucson (USA)
A quantitative method was developed for the assay of vitamin K in human colostrum and milk. The procedure combines preparative and analytical chromatography on silica gel in a nitrogen atmosphere followed by reversed phase high performance liquid chromatography (HPLC). Two HPLC steps were used: gradient separation with ultraviolet (UV) detection followed by isocratic separation detected electrochemically. Due to co-migrating impurities, UV detection alone is insufficient for identification of vitamin K. Exogenous vitamin K was shown to equilibrate with endogenous vitamin K in the samples. A statistical method was incorporated to control for experimental variability. Vitamin K1 was analyzed in 16 pooled milk samples from 7 donors and in individual samples from 15 donors at 1 month post-partum. Vitamin K1 was present at 2.94 +/- 1.94 and 3.15 +/- 2.87 ng/mL in pools and in individuals, respectively. Menaquinones, the bacterial form of the vitamin, were not detected. The significance of experimental variation to studies of vitamin K in individuals is discussed.
- OSTI ID:
- 6511751
- Journal Information:
- Lipids; (USA), Vol. 25:7; ISSN 0024-4201
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MILK
CHEMICAL COMPOSITION
VITAMIN K
BIOASSAY
LIQUID COLUMN CHROMATOGRAPHY
SCINTILLATION COUNTING
ULTRAVIOLET RADIATION
AROMATICS
BIOLOGICAL MATERIALS
BODY FLUIDS
CHROMATOGRAPHY
COAGULANTS
COUNTING TECHNIQUES
DRUGS
ELECTROMAGNETIC RADIATION
FOOD
HEMATOLOGIC AGENTS
HEPARIN ANTAGONISTS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
QUINONES
RADIATIONS
SEPARATION PROCESSES
VITAMINS
550201* - Biochemistry- Tracer Techniques