Associations of Escherichia coli K-12 OmpF trimers with rough and smooth lipopolysaccharides
- Louisiana State Univ. Medical Center, New Orleans (USA)
The associations of both rough and smooth lipopolysaccharides (LPS) with the OmpF porin of Escherichia coli K-12 were examined in galE strains deleted for ompC. Transformation with pSS37 and growth with galactose conferred the ability to assemble a Shigella dysenteriae O antigen onto the core oligosaccharide of E. coli K-12 LPS. The association of LPS with OmpF trimers was assessed by staining, autoradiography of LPS specifically labeled with (1-14C)galactose, and Western immunoblotting with a monoclonal antibody specific for OmpF trimers. These techniques revealed that the migration distances and multiple banding patterns of OmpF porin trimers in sodium dodecyl sulfate-polyacrylamide gels were dictated by the chemotype of associated LPS. Expression of smooth LPS caused almost all of the trimeric OmpF to run in gels with a slower mobility than trimers from rough strains. The LPS associated with trimers from a smooth strain differed from the bulk-phase LPS by consisting almost exclusively of molecules with O antigen.
- OSTI ID:
- 6511251
- Journal Information:
- Journal of Bacteriology; (USA), Vol. 172:9; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
Similar Records
Surface protein composition of Aeromonas hydrophila strains virulent for fish: identification of a surface array protein
Epitope identification for a panel of anti-Sinorhizobium meliloti monoclonal antibodies and application to the analysis of K antigens and lipopolysaccharides from bacteroids
Related Subjects
ESCHERICHIA COLI
GROWTH
LIPOPOLYSACCHARIDES
AUTORADIOGRAPHY
CARBON 14 COMPOUNDS
PORINS
TWO-DIMENSIONAL ELECTROPHORESIS
BACTERIA
CARBOHYDRATES
ELECTROPHORESIS
LABELLED COMPOUNDS
LIPIDS
MEMBRANE PROTEINS
MICROORGANISMS
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques