Genome shortcut leads to problems

Anderson, C

doi:10.1126/science.8456291

Title: Genome shortcut leads to problems

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Abstract

Mega YACs (yeast artificial chromosomes), which can carry DNA sequences up to 1.4 million bases long, were anticipated as a major for mapping the human genome. They have been found to have as much as 80% chimerism, however, and contain many deletions and rearrangements. This makes them useless for high-resolution mapping, but they are effective for connecting points over long distances. Mega YACs are still useful for mapping 95% of the human genome.

Authors:: Anderson, C

Publication Date:: Fri Mar 19 00:00:00 EST 1993

OSTI Identifier:: 6501890

Resource Type:: Journal Article

Journal Name:: Science (Washington, D.C.); (United States)

Additional Journal Information:: Journal Volume: 259:5102; Journal ID: ISSN 0036-8075

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; GENES; DNA-CLONING; HUMAN CHROMOSOMES; MAPPING; CHROMOSOMES; COSMIDS; DNA SEQUENCING; HUMAN CHROMOSOME 17; HUMAN CHROMOSOME 21; YEASTS; CLONING; DNA HYBRIDIZATION; EUMYCOTA; FUNGI; HYBRIDIZATION; MICROORGANISMS; PLANTS; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics

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                    Anderson, C. Genome shortcut leads to problems.  United States: N. p., 1993. 
        Web.  doi:10.1126/science.8456291.

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                    Anderson, C. Genome shortcut leads to problems.  United States.  https://doi.org/10.1126/science.8456291

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                    Anderson, C. 1993.  
        "Genome shortcut leads to problems".  United States.  https://doi.org/10.1126/science.8456291.

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@article{osti_6501890,

  title        = {Genome shortcut leads to problems},

  author       = {Anderson, C},

  abstractNote = {Mega YACs (yeast artificial chromosomes), which can carry DNA sequences up to 1.4 million bases long, were anticipated as a major for mapping the human genome. They have been found to have as much as 80% chimerism, however, and contain many deletions and rearrangements. This makes them useless for high-resolution mapping, but they are effective for connecting points over long distances. Mega YACs are still useful for mapping 95% of the human genome.},

  doi          = {10.1126/science.8456291},

  url          = {https://www.osti.gov/biblio/6501890},
  journal      = {Science (Washington, D.C.); (United States)},

  issn         = {0036-8075},

  number       = ,

  volume       = 259:5102,

  place        = {United States},

  year         = {Fri Mar 19 00:00:00 EST 1993},

  month        = {Fri Mar 19 00:00:00 EST 1993}

}

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