Scintillation proximity assay: a sensitive and continuous isotopic method for monitoring ligand/receptor and antigen/antibody interactions
Scintillation proximity assay (SPA) makes it possible to use radioisotopes for monitoring binding reactions continuously without the need to separate free from bound components. As a result SPA can be carried out more rapidly than most other methods used to monitor binding reactions. The methodology also lends itself to automation. The sensitivities already achieved with SPA procedures are comparable to the sensitivities of other procedures in use today. Another feature of SPA is that the key reagents (beads, /sup 125/I labeling) are relatively inexpensive. The principles of SPA, utilizing /sup 125/I-labeled molecules, are discussed and some applications to immunology, receptor binding, and measurement of potential across membranes are presented. SPA should also be applicable to monitoring interactions involving nucleic acids, lipids, and carbohydrates. Characteristics of some radionuclides, other than tritium and /sup 125/I, that may be used in SPA are presented.
- Research Organization:
- Roche Research Center, Nutley, NJ
- OSTI ID:
- 6501311
- Journal Information:
- Anal. Biochem.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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RADIOIMMUNOASSAY
EVALUATION
ANTIGEN-ANTIBODY REACTIONS
BIOCHEMICAL REACTION KINETICS
FLUORESCENCE
IODINE 125
LIGANDS
TRITIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LUMINESCENCE
NUCLEI
ODD-EVEN NUCLEI
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques