Conformational changes of fibronectin induced by polystyrene derivatives with a heparin-like function
- CHU Necker-Enfants Malades, Paris (France)
- Univ. Paris-Nord, Villetaneuse (France)
- IRSC, Villejuif (France)
It was previously reported that polystyrene substituted with the sulfonate group, PSSO[sub 3], which has anticoagulant heparin-like properties, and then coated with fibronectin supports the growth of human umbilical vein endothelial cells. On the other hand, polystyrene substituted with the amino acid sulfamide group, PSSO[sub 2]-Asp, which has a higher anticoagulant activity, and then coated with fibronectin no longer supported the growth of endothelial cells. The authors report here that, while the affinity of fibronectin to either polymer is of the same order of magnitude, fibronectin is adsorbed onto the PSSO[sub 2]-Asp polymer in a different conformation compared to the PSSO[sub 3] polymer. This was shown by a higher binding of polyclonal antifibronectin antibodies to fibronectin-coated PSSO[sub 2]-Asp polymer, and by a decreased susceptibility of the coated fibronectin to proteolysis by thermolysin. This study provides evidence that a solid phase substrate with a strong heparin-like function may influence the conformation and biological properties of fibronectin.
- OSTI ID:
- 6500885
- Journal Information:
- Journal of Biomedical Materials Research; (United States), Vol. 27:5; ISSN 0021-9304
- Country of Publication:
- United States
- Language:
- English
Similar Records
Structural and functional aspects of phosphorylation of human fibronectins
Single-molecule dynamic force spectroscopy of the fibronectin-heparin interaction
Related Subjects
POLYSTYRENE
TOXICITY
PROTEINS
ULTRASTRUCTURAL CHANGES
CHEMICAL COMPOSITION
MAN
PROSTHESES
ANIMALS
MAMMALS
MATERIALS
MEDICAL SUPPLIES
MORPHOLOGICAL CHANGES
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
PLASTICS
POLYMERS
POLYOLEFINS
POLYVINYLS
PRIMATES
SYNTHETIC MATERIALS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology