Quantitative measurements of regional glucose utilization and rate of valine incorporation into proteins by double-tracer autoradiography in the rat brain tumor model

Journal Article · Wed Feb 01 00:00:00 EST 1989 · J. Cereb. Blood Flow Metab.; (United States)

DOI:https://doi.org/10.1038/jcbfm.1989.12· OSTI ID:6499575

Kirikae, M; Diksic, M; Yamamoto, Y L

We examined the rate of glucose utilization and the rate of valine incorporation into proteins using 2-(/sup 18/F)fluoro-2-deoxyglucose and L-(1-14C)-valine in a rat brain tumor model by quantitative double-tracer autoradiography. We found that in the implanted tumor the rate of valine incorporation into proteins was about 22 times and the rate of glucose utilization was about 1.5 times that in the contralateral cortex. (In the ipsilateral cortex, the tumor had a profound effect on glucose utilization but no effect on the rate of valine incorporation into proteins.) Our findings suggest that it is more useful to measure protein synthesis than glucose utilization to assess the effectiveness of antitumor agents and their toxicity to normal brain tissue. We compared two methods to estimate the rate of valine incorporation: kinetic (quantitation done using an operational equation and the average brain rate coefficients) and washed slices (unbound labeled valine removed by washing brain slices in 10% trichloroacetic acid). The results were the same using either method. It would seem that the kinetic method can thus be used for quantitative measurement of protein synthesis in brain tumors and normal brain tissue using (/sup 11/C)-valine with positron emission tomography.

• Research Organization: McGill Univ., Quebec (Canada)
• OSTI ID: 6499575
• Journal Information: J. Cereb. Blood Flow Metab.; (United States), Vol. 9:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRAIN
NEOPLASMS
GLUCOSE
METABOLISM
PROTEINS
BIOSYNTHESIS
VALINE
AUTORADIOGRAPHY
BIOLOGICAL MODELS
CARBON 14 COMPOUNDS
DOUBLE LABELLING
FLUORINE 18
RATS
ALDEHYDES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DISEASES
FLUORINE ISOTOPES
HEXOSES
HOURS LIVING RADIOISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MAMMALS
MONOSACCHARIDES
NERVOUS SYSTEM
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
RODENTS
SACCHARIDES
SYNTHESIS
VERTEBRATES
550501* - Metabolism- Tracer Techniques