Ultraviolet B irradiation induces changes in the distribution and release of arachidonic acid, dihomo-gamma-linolenic acid, and eicosapentaenoic acid in human keratinocytes in culture
There is increasing evidence that derivatives of 20-carbon polyunsaturated fatty acids, the eicosanoids, play an important role in the inflammatory responses of the human skin. To better understand the metabolic fate of fatty acids in the skin, the effect of ultraviolet B (UVB) irradiation (280-320 nm) on the distribution and release of /sup 14/C-labeled arachidonic acid, dihomo-gamma-linolenic acid, and eicosapentaenoic acid in human keratinocytes in culture was investigated. Ultraviolet B irradiation induced the release of all three /sup 14/C-labeled fatty acids from the phospholipids, especially from phosphatidylethanolamine, and this was accompanied by increased labeling of the nonphosphorus lipids. This finding suggests that UVB induces a significant liberation of eicosanoid precursor fatty acids from cellular phospholipids, but the liberated fatty acids are largely reincorporated into the nonphosphorus lipids. In conclusion, the present study suggests that not only arachidonic acid but also dihomo-gamma-linolenic acid, and eicosapentaenoic acid might be involved in the UVB irradiation-induced inflammatory reactions of human skin.
- Research Organization:
- Univ. of Turku, Finland
- OSTI ID:
- 6498740
- Journal Information:
- J. Invest. Dermatol.; (United States), Vol. 5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
ARACHIDONIC ACID
TISSUE DISTRIBUTION
EICOSANOIC ACID
LINOLENIC ACID
SKIN
INFLAMMATION
CARBON 14 COMPOUNDS
CELL CULTURES
EPIDERMIS
KERATIN
LIPIDS
MAN
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
DISTRIBUTION
ELECTROMAGNETIC RADIATION
EPITHELIUM
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PRIMATES
PROTEINS
RADIATION EFFECTS
RADIATIONS
SCLEROPROTEINS
SYMPTOMS
TISSUES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture