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Title: Pulmonary gallium uptake in rats with granulomatosis induced by complete Freund adjuvant

Abstract

To investigate the mechanism of gallium-67 uptake in lung granulomatosis, we studied 13 rats in which lung granulomatosis was induced by injection of complete Freund adjuvant (CFA) and 14 controls. Gallium uptake was assessed in bronchoalveolar lavage fluid and lavaged lung. The cells responsible for gallium uptake were identified by latent image activation autoradiography. Gallium activity in both lavaged lungs and bronchoalveolar cells (BAC) was higher in CFA-treated animals than in controls (172,205 +/- 134,783 DPM versus 44,456 +/- 14,486 DPM +/- SD (p less than 0.05) and 40,083 +/- 16,350 DPM versus 9100 +/- 4114 DPM (p less than 0.05), respectively). In control rats, about two-thirds of total lung gallium was located in the interstitium, whereas in CFA-treated rats it was found in the mononuclear cells of lung granulomas. Gallium tracks were more numerous in the alveolar macrophages (AM) of CFA-treated rats than in control AM (28.4 +/- 10.0/field versus 8.4 +/- 3.8/field, p less than 0.001) but the number of tracks was proportional to the number of AM (52.4 +/- 18.7 versus 12.2 +/- 4.3, respectively; p less than 0.001). It is concluded that in rats with CFA-induced lung granulomatosis 1) pulmonary gallium uptake increases, 2) mononuclear cellsmore » are responsible for this uptake in both granulomas and AM, and 3) the increased uptake is due to the increased number of mononuclear cells.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Universite Rene Descartes, Paris (France)
OSTI Identifier:
6498704
Resource Type:
Journal Article
Journal Name:
Exp. Lung Res.; (United States)
Additional Journal Information:
Journal Volume: 14:4
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; GALLIUM 67; UPTAKE; GRANULOMAS; DIAGNOSIS; LUNGS; AUTORADIOGRAPHY; BIOLOGICAL MODELS; LAVAGE; MACROPHAGES; MONOCYTES; RATS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; GALLIUM ISOTOPES; INTERMEDIATE MASS NUCLEI; ISOTOPES; LEUKOCYTES; MAMMALS; MATERIALS; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; ORGANS; PHAGOCYTES; RADIOISOTOPES; RESPIRATORY SYSTEM; RODENTS; SOMATIC CELLS; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Stanislas-Leguern, G, Masse, R, Jaubert, F, Chretien, J, and Huchon, G. Pulmonary gallium uptake in rats with granulomatosis induced by complete Freund adjuvant. United States: N. p., 1988. Web. doi:10.3109/01902148809087820.
Stanislas-Leguern, G, Masse, R, Jaubert, F, Chretien, J, & Huchon, G. Pulmonary gallium uptake in rats with granulomatosis induced by complete Freund adjuvant. United States. https://doi.org/10.3109/01902148809087820
Stanislas-Leguern, G, Masse, R, Jaubert, F, Chretien, J, and Huchon, G. 1988. "Pulmonary gallium uptake in rats with granulomatosis induced by complete Freund adjuvant". United States. https://doi.org/10.3109/01902148809087820.
@article{osti_6498704,
title = {Pulmonary gallium uptake in rats with granulomatosis induced by complete Freund adjuvant},
author = {Stanislas-Leguern, G and Masse, R and Jaubert, F and Chretien, J and Huchon, G},
abstractNote = {To investigate the mechanism of gallium-67 uptake in lung granulomatosis, we studied 13 rats in which lung granulomatosis was induced by injection of complete Freund adjuvant (CFA) and 14 controls. Gallium uptake was assessed in bronchoalveolar lavage fluid and lavaged lung. The cells responsible for gallium uptake were identified by latent image activation autoradiography. Gallium activity in both lavaged lungs and bronchoalveolar cells (BAC) was higher in CFA-treated animals than in controls (172,205 +/- 134,783 DPM versus 44,456 +/- 14,486 DPM +/- SD (p less than 0.05) and 40,083 +/- 16,350 DPM versus 9100 +/- 4114 DPM (p less than 0.05), respectively). In control rats, about two-thirds of total lung gallium was located in the interstitium, whereas in CFA-treated rats it was found in the mononuclear cells of lung granulomas. Gallium tracks were more numerous in the alveolar macrophages (AM) of CFA-treated rats than in control AM (28.4 +/- 10.0/field versus 8.4 +/- 3.8/field, p less than 0.001) but the number of tracks was proportional to the number of AM (52.4 +/- 18.7 versus 12.2 +/- 4.3, respectively; p less than 0.001). It is concluded that in rats with CFA-induced lung granulomatosis 1) pulmonary gallium uptake increases, 2) mononuclear cells are responsible for this uptake in both granulomas and AM, and 3) the increased uptake is due to the increased number of mononuclear cells.},
doi = {10.3109/01902148809087820},
url = {https://www.osti.gov/biblio/6498704}, journal = {Exp. Lung Res.; (United States)},
number = ,
volume = 14:4,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}