Detection of human cytomegalovirus by slot-blot hybridization assay employing oligo-primed /sup 32/P-labelled probe
A /sup 32/P-labelled Hind III-0 DNA fragment (nine Kilobases; Kb) from human cytomegalovirus AD-169 (HCMV) was used in slot-blot hybridization assay for the detection of HCMV in clinical samples. The results obtained with DNA hybridization assay (DNA HA) were compared with virus isolation using conventional tube cell culture (CTC) and centrifugation vial culture (CVC), immunofluorescence (IF), and complement fixation test (CFT). Of 15 CTC-positive samples, 13 were positive with DNA HA (sensitivity 86.7%). Also, 14 additional samples were DNA HA-positive but CTC-negative. CVC and/or IF confirmed the diagnosis in nine of 14; the remaining five samples were from three patients who showed fourfold rising antibody titre by CFT. Although DNA HA using /sup 32/P-labelled probes is relatively cumbersome and expensive, it is a valuable test for quantitation of viral shedding in patients with HCMV infections who may benefit from antiviral therapy.
- Research Organization:
- Charing Cross and Westminister Medical School, London (England)
- OSTI ID:
- 6497458
- Journal Information:
- J. Med. Virol.; (United States), Vol. 26:4
- Country of Publication:
- United States
- Language:
- English
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DNA
HYBRIDIZATION
VIRUSES
BIOASSAY
CELL CULTURES
COMPLEMENT
FLUORESCENCE
MAN
PHOSPHORUS 32
SENSITIVITY ANALYSIS
SPECIFICITY
TRACER TECHNIQUES
VIRAL DISEASES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LUMINESCENCE
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
VERTEBRATES
550901* - Pathology- Tracer Techniques