Induction of contact drematitis in guinea pigs by quaternary ammonium compounds: the mechanisms of antigen formation
Eight quaternary ammonium compounds were tested for their ability to induce contact dermatitis in guinea pigs by using a modified Freund's complete adjuvant test together with the guinea pig maximization test. Only two quaternary ammonium salts of eight tested could be designated as strong allergens. These two active substances were shown to be capable of stable association with membrane lipids in forming immunogenic complexes. This surface complexation phenomenon was confirmed by using a spin-labeled quaternary ammonium salt which competed for binding sites to the surface of epidermal cells in vivo. Electron spin resonance was used to demonstrate that stable ion-pairs are formed between binding sites and the two allergenic preservatives. Furthermore, information was obtained on the kinetics of immunogenic complex formation as well as on the position and orientation of the quaternary ammonium ion at the cell surface.
- Research Organization:
- Univ. of Minnesota, MN
- OSTI ID:
- 6486211
- Journal Information:
- Environ. Health Perspect.; (United States), Vol. 70
- Country of Publication:
- United States
- Language:
- English
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QUATERNARY COMPOUNDS
BIOLOGICAL EFFECTS
ANTIGENS
DERMATITIS
ELECTRON SPIN RESONANCE
GUINEA PIGS
HEAVY WATER
HISTOLOGY
NMR SPECTRA
SKIN
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BODY
DISEASES
HYDROGEN COMPOUNDS
MAGNETIC RESONANCE
MAMMALS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
RESONANCE
RODENTS
SKIN DISEASES
SPECTRA
VERTEBRATES
WATER
560300* - Chemicals Metabolism & Toxicology