Evidence for localized cell heating induced by infrared optical tweezers
- Department of Electrical and Computer Engineering and Beckman Laser Institute and Medical Clinic, University of California, Irvine, California 92717 (United States)
- Department of Biophysics, and Beckman Laser Institute and Medical Clinic, University of California, Irvine, California 92717 (United States)
The confinement of liposomes and Chinese hamster ovary (CHO) cells by infrared (IR) optical tweezers is shown to result in sample heating and temperature increases by several degrees centigrade, as measured by a noninvasive, spatially resolved fluorescence detection technique. For micron-sized spherical liposome vesicles having bilayer membranes composed of the phospholipid 1,2-diacyl-pentadecanoyl-glycero-phosphocholine (15-OPC), a temperature rise of {similar_to}1.45{plus_minus}0.15 {degree}C/100 mW is observed when the vesicles are held stationary with a 1.064 {mu}m optical tweezers having a power density of {similar_to}10{sup 7} W/cm{sup 2} and a focused spot size of {similar_to}0.8 {mu}m. The increase in sample temperature is found to scale linearly with applied optical power in the 40 to 250 mW range. Under the same trapping conditions, CHO cells exhibit an average temperature rise of nearly 1.15{plus_minus}0.25 {degree}C/100 mW. The extent of cell heating induced by infrared tweezers confinement can be described by a heat conduction model that accounts for the absorption of infrared (IR) laser radiation in the aqueous cell core and membrane regions, respectively. The observed results are relevant to the assessment of the noninvasive nature of infrared trapping beams in micromanipulation applications and cell physiological studies. {copyright} {ital 1995} {ital American} {ital Institute} {ital of} {ital Physics}.
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG03-91ER61227
- OSTI ID:
- 64859
- Journal Information:
- Biophysical Journal, Vol. 68, Issue 5; Other Information: PBD: May 1995
- Country of Publication:
- United States
- Language:
- English
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