Use of fish gill cells in culture to evaluate the cytotoxicity and photocytotoxicity of intact and photomodified creosote
- Univ. of Waterloo, Ontario (Canada). Dept. of Biology
The influence of ultraviolet (UV) irradiation on creosote toxicity was investigated with the rainbow trout (Oncorhynchus mykiss) gill cell line, RTgill-W1, and two indicator dyes, alamar Blue[trademark] and 5-carboxyfluorescein diacetate acetoxymethyl ester. These monitor redox potential and membrane integrity, respectively. After solubilization and chemical analysis, creosote was presented to cells in the dark to measure cytotoxicity or concurrently with UV irradiation to evaluate photocytotoxicity. Additionally, creosote was photomodified by 2 h of UV irradiation before presentation to cells in the dark or together with UV. Cytotoxicity was detected only at high nominal creosote concentrations, but photocytotoxicity occurred at creosote concentrations 35-fold lower. All the aromatic hydrocarbons in creosote appeared to contribute to cytotoxicity, but photocytotoxicity was due only to the fluoranthene, pyrene, anthracene, and benzo[a]anthracene in the mixture. Photomodified creosote was much more cytotoxic than intact creosote and this difference was most pronounced in the alamar Blue assay. Likely, this was due to photomodification products that impaired the mitochondrial electron transport chain. Photomodified creosote was slightly less photocytotoxic than intact creosote. Overall these results indicate that UV irradiation potentially enhances the toxicity of creosote to fish in several different but significant ways.
- OSTI ID:
- 6480846
- Journal Information:
- Environmental Toxicology and Chemistry, Vol. 18:6; ISSN 0730-7268
- Country of Publication:
- United States
- Language:
- English
Similar Records
Impacts of UV radiation and photomodification on the toxicity of PAHs to the higher plant Lemna gibba (duckweed)
Effects of simulated solar radiation on the bioaccumulation of polycyclic aromatic hydrocarbons by the duckweed, Lemna gibba
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOASSAY
BIOLOGICAL INDICATORS
BIOLOGICAL MARKERS
CELL CULTURES
CREOSOTE
FISHES
GILLS
POLYCYCLIC AROMATIC HYDROCARBONS
ULTRAVIOLET RADIATION
WATER POLLUTION
ANIMALS
AQUATIC ORGANISMS
AROMATICS
ELECTROMAGNETIC RADIATION
HYDROCARBONS
ORGANIC COMPOUNDS
POLLUTION
RADIATIONS
RESPIRATORY SYSTEM
VERTEBRATES
540320* - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)
560300 - Chemicals Metabolism & Toxicology