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Title: Chloroplast-targeted ERD1 protein declines but its mRNA increases during senescence in Arabidopsis

Abstract

Arabidopsis ERD1 is a ClpC-like protein that sequence analysis suggests may interact with the chloroplast-localized ClpP protease to facilitate proteolysis. The mRNA encoded by the ERD1 gene has previously been shown to accumulate in response to senescence and to a variety of stresses and hormones. Here the authors show that the ERD1 protein, in contrast to the ERD1 mRNA, strongly declines in abundance with age, becoming undetectable in fully expanded leaves. Sequence analysis also suggests that ERD1 is chloroplast targeted, and they show in an in vitro system that the native protein is properly imported, processed, and present within the soluble fraction of the chloroplast, presumably the stroma. They show that ClpP protein, which is also present in the stroma, declines with age in parallel with ERD1. These results are consistent with the interaction of ERD1 and ClpP, but they suggest that it is unlikely that either plays a major role during senescence. Certain other chloroplast proteins decline with age coordinately with ERD1 and ClpP, suggesting that these declines are markers of an early age-mediated change that occurs within the chloroplast.

Authors:
;  [1];  [2]
  1. Univ. of Wisconsin, Madison, WI (United States). Dept. of Biochemistry
  2. Michigan State Univ., East Lansing, MI (United States). DOE Plant Research Lab.
Publication Date:
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
6479802
DOE Contract Number:  
FG02-97ER20280
Resource Type:
Journal Article
Journal Name:
Plant Physiology; (Bethesda)
Additional Journal Information:
Journal Volume: 119:4; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AGE DEPENDENCE; ARABIDOPSIS; BIOLOGICAL FUNCTIONS; CHLOROPLASTS; GENES; MESSENGER-RNA; PEPTIDE HYDROLASES; PROTEINS; PROTEOLYSIS; CELL CONSTITUENTS; CHEMICAL REACTIONS; DECOMPOSITION; ENZYMES; HYDROLASES; MAGNOLIOPHYTA; MAGNOLIOPSIDA; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PLANTS; RNA; 550200* - Biochemistry

Citation Formats

Weaver, L M, Amasino, R M, and Froehlich, J E. Chloroplast-targeted ERD1 protein declines but its mRNA increases during senescence in Arabidopsis. United States: N. p., 1999. Web. doi:10.1104/pp.119.4.1209.
Weaver, L M, Amasino, R M, & Froehlich, J E. Chloroplast-targeted ERD1 protein declines but its mRNA increases during senescence in Arabidopsis. United States. https://doi.org/10.1104/pp.119.4.1209
Weaver, L M, Amasino, R M, and Froehlich, J E. 1999. "Chloroplast-targeted ERD1 protein declines but its mRNA increases during senescence in Arabidopsis". United States. https://doi.org/10.1104/pp.119.4.1209.
@article{osti_6479802,
title = {Chloroplast-targeted ERD1 protein declines but its mRNA increases during senescence in Arabidopsis},
author = {Weaver, L M and Amasino, R M and Froehlich, J E},
abstractNote = {Arabidopsis ERD1 is a ClpC-like protein that sequence analysis suggests may interact with the chloroplast-localized ClpP protease to facilitate proteolysis. The mRNA encoded by the ERD1 gene has previously been shown to accumulate in response to senescence and to a variety of stresses and hormones. Here the authors show that the ERD1 protein, in contrast to the ERD1 mRNA, strongly declines in abundance with age, becoming undetectable in fully expanded leaves. Sequence analysis also suggests that ERD1 is chloroplast targeted, and they show in an in vitro system that the native protein is properly imported, processed, and present within the soluble fraction of the chloroplast, presumably the stroma. They show that ClpP protein, which is also present in the stroma, declines with age in parallel with ERD1. These results are consistent with the interaction of ERD1 and ClpP, but they suggest that it is unlikely that either plays a major role during senescence. Certain other chloroplast proteins decline with age coordinately with ERD1 and ClpP, suggesting that these declines are markers of an early age-mediated change that occurs within the chloroplast.},
doi = {10.1104/pp.119.4.1209},
url = {https://www.osti.gov/biblio/6479802}, journal = {Plant Physiology; (Bethesda)},
issn = {0032-0889},
number = ,
volume = 119:4,
place = {United States},
year = {Thu Apr 01 00:00:00 EST 1999},
month = {Thu Apr 01 00:00:00 EST 1999}
}